Regulation of fowl sperm flagellar motility by protein phosphatase type 1 and its relationship with dephosphorylation of axonemal and/or accessory cytoskeletal proteins

Biochemical and Biophysical Research Communications
K AshizawaY Tsuzuki

Abstract

The motility of demembranated fowl spermatozoa was vigorous at 30 degrees C in the presence of ATP, but decreased markedly following the addition of recombinant protein phosphatase type 1 (PP-1) supplemented with Mn2+. This inhibition was not restored by the addition of cAMP, within the range 1-1000 microM, but instantly restored by the addition of 50 ng/ml trypsin. Phosphorylation of demembranated fowl sperm proteins during incubation with [gamma-32P]ATP at 30 degrees C was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A marked difference in phosphorylation status was observed in approximately 116, 86, 79, 50 and 29-kDa proteins. These proteins were dephosphorylated in the presence of PP-1 and Mn2+ compared with those in control samples. These results suggest that PP-1-mediated dephosphorylation of some of these proteins of the axoneme and/or accessory cytoskeletal components of fowl spermatozoa may be involved in the inhibition of motility.

References

Nov 1, 1991·Journal of Reproduction and Fertility·M F Thomson, G J Wishart
Jan 1, 1990·Archives of Andrology·G C MajumderM Barua
May 1, 1989·Journal of Reproduction and Fertility·K AshizawaK Okauchi
Jan 1, 1989·Annual Review of Biochemistry·P Cohen
Jan 1, 1989·Cell Motility and the Cytoskeleton·J S Tash
Jan 1, 1989·Archives of Andrology·C B Lindemann, K S Kanous
May 1, 1989·Journal of Reproduction and Fertility·K AshizawaK Okauchi
Jul 1, 1987·Journal of Reproduction and Fertility·G J Wishart, K Ashizawa
Nov 1, 1987·Journal of Cellular Biochemistry·C J Brokaw
Jan 1, 1986·Cell Motility and the Cytoskeleton·H MurofushiH Sakai
Feb 1, 1981·Fertility and Sterility·D F Katz, J W Overstreet
Mar 1, 1996·Biology of Reproduction·G D SmithS Vijayaraghavan

❮ Previous
Next ❯

Citations

Dec 8, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·K AshizawaY Tsuzuki
Jan 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·K AshizawaY Tsuzuki
Oct 3, 2002·Biochemical and Biophysical Research Communications·Kazuo Inaba
May 7, 1999·Molecular Reproduction and Development·K U DeviS Shivaji

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.