Regulation of fowl sperm flagellar motility by protein phosphatase type 1 and its relationship with dephosphorylation of axonemal and/or accessory cytoskeletal proteins

Biochemical and Biophysical Research Communications
K AshizawaY Tsuzuki


The motility of demembranated fowl spermatozoa was vigorous at 30 degrees C in the presence of ATP, but decreased markedly following the addition of recombinant protein phosphatase type 1 (PP-1) supplemented with Mn2+. This inhibition was not restored by the addition of cAMP, within the range 1-1000 microM, but instantly restored by the addition of 50 ng/ml trypsin. Phosphorylation of demembranated fowl sperm proteins during incubation with [gamma-32P]ATP at 30 degrees C was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A marked difference in phosphorylation status was observed in approximately 116, 86, 79, 50 and 29-kDa proteins. These proteins were dephosphorylated in the presence of PP-1 and Mn2+ compared with those in control samples. These results suggest that PP-1-mediated dephosphorylation of some of these proteins of the axoneme and/or accessory cytoskeletal components of fowl spermatozoa may be involved in the inhibition of motility.


Nov 1, 1991·Journal of Reproduction and Fertility·M F Thomson, G J Wishart
Jan 1, 1990·Archives of Andrology·G C MajumderM Barua
May 1, 1989·Journal of Reproduction and Fertility·K AshizawaK Okauchi
Jan 1, 1989·Annual Review of Biochemistry·P Cohen
Jan 1, 1989·Cell Motility and the Cytoskeleton·J S Tash
Jan 1, 1989·Archives of Andrology·C B Lindemann, K S Kanous
May 1, 1989·Journal of Reproduction and Fertility·K AshizawaK Okauchi
Jul 1, 1987·Journal of Reproduction and Fertility·G J Wishart, K Ashizawa
Nov 1, 1987·Journal of Cellular Biochemistry·C J Brokaw
Jan 1, 1986·Cell Motility and the Cytoskeleton·H MurofushiH Sakai
Feb 1, 1981·Fertility and Sterility·D F Katz, J W Overstreet
Mar 1, 1996·Biology of Reproduction·G D SmithS Vijayaraghavan

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Dec 8, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·K AshizawaY Tsuzuki
Jan 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·K AshizawaY Tsuzuki
Oct 3, 2002·Biochemical and Biophysical Research Communications·Kazuo Inaba
May 7, 1999·Molecular Reproduction and Development·K U DeviS Shivaji

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