PMID: 9418241Jan 7, 1998Paper

Regulation of fumarase (fumB) gene expression in Escherichia coli in response to oxygen, iron and heme availability: role of the arcA, fur, and hemA gene products

FEMS Microbiology Letters
C P Tseng

Abstract

Three distinct fumarases, FumA, FumB and FumC, have been reported in Escherichia coli. While the fumA and fumC gene products are expressed under aerobic cell growth conditions, the FumB fumarase appears to be more abundant during anaerobic growth. To study the transcriptional regulation of the fumB gene, a fumB-lacZ operon fusion was constructed and analyzed in a single copy under a variety of cell culture conditions. Expression of fumB-lacZ was fourfold higher under anaerobic than aerobic growth conditions. This anaerobic response is modulated by the ArcA and Fnr proteins, which function independently as anaerobic activators of fumB gene expression. Cellular iron limitation in a fur mutant caused fumB-lacZ expression to decrease sevenfold while cellular heme limitation decreased fumB gene expression twofold. In addition, fumB-lacZ expression was shown to vary depending on the DNA superhelicity. This study further delineates the regulation of the fumB gene in cell growth.

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Citations

Jul 4, 2012·The Journal of Microbiology·Yu-Pei ChenChing-Ping Tseng
Oct 9, 2012·BMC Evolutionary Biology·Olga V TsoyMikhail S Gelfand
Jan 15, 2010·Molecular Microbiology·Sylvain Durand, Gisela Storz
Dec 18, 2013·Biotechnology and Bioengineering·Faraz BaigSarah W Harcum
May 20, 2003·The Journal of Biological Chemistry·Kirsty SalmonRobert P Gunsalus
May 15, 2003·The Journal of Biological Chemistry·Jonathan P McHughSimon C Andrews
Jul 22, 2019·Applied and Environmental Microbiology·Zhongmei LiuZhemin Zhou
Apr 6, 2018·Biotechnology for Biofuels·Zaigao TanJacqueline V Shanks
Dec 13, 2018·Nucleic Acids Research·Masatoshi MiyakoshiJörg Vogel
May 15, 2002·The Journal of Biological Chemistry·Markus W Covert, Bernhard Ø Palsson
Feb 26, 2020·Microbial Cell Factories·Lei ZhaoXiaoyuan Wang
Jan 31, 2020·Frontiers in Bioengineering and Biotechnology·Jean Marie FrançoisNicolas Morin
Feb 9, 2005·The Journal of Biological Chemistry·Kirsty A SalmonRobert P Gunsalus
Apr 24, 2015·Applied Microbiology and Biotechnology·Ting ZhangYajun Yan
Jan 20, 2007·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Kazuya MorikawaToshiko Ohta
Jun 28, 2003·FEMS Microbiology Reviews·Simon C AndrewsFrancisco Rodríguez-Quiñones
Jun 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Yardena SilasOphry Pines

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