Regulation of G protein-mediated adenylyl cyclase in striatum and cortex of opiate-dependent and opiate withdrawing mice

Brain Research
G B KaplanK A Leite-Morris

Abstract

Previous research has demonstrated that acute and chronic opiate treatment alters receptor- and postreceptor-mediated adenylyl cyclase activity. This study examined the regulation of G protein- and forskolin-mediated adenylyl cyclase activity in mouse striatum and cortex after short- and long-term opiate exposure. To directly measure adenylyl cyclase enzymatic activity, assays were done in the presence of catalytic site activator forskolin. To measure G protein-mediated adenylyl cyclase activity, assays were performed in the presence of non-hydrolyzable guanosine 5'-triphosphate (GTP) analogue, 5'-guanylyl-imidodiphosphate. Short-term in vitro morphine exposure produced reductions in forskolin-stimulated adenylyl cyclase activity in striatal and cortical tissues. Long-term morphine treatment in mice was performed via morphine- or placebo-pellet implantation for 72 h; this treatment has been shown to produce opiate dependence and withdrawal. In both opiate-dependent and opiate withdrawing mice (1 h post-naloxone induction), there were significant increases in G protein-mediated adenylyl cyclase activity in the striatum (vs. controls). In opiate-dependent mice, there was an decrease in G protein-mediated adenylyl cyclase activity...Continue Reading

Citations

Mar 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Daniela SachsSérgio H Ferreira
Aug 12, 2010·Behavioural Pharmacology·Michael Scott Bowers
Oct 19, 2006·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Elizabeth M ByrnesJohn J Byrnes
Nov 19, 2005·Peptides·Bhagya L PuppalaAnil Gulati

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