PMID: 9657444Jul 10, 1998Paper

Regulation of gelatinase activity in mice with targeted inactivation of components of the plasminogen/plasmin system

Thrombosis and Haemostasis
H R LijnenD Collen

Abstract

To investigate a potential physiological role of the plasminogen/plasmin system in activation of the matrix metalloproteinase (MMP) system, the distribution of latent and active MMP-2 (gelatinase A) or MMP-9 (gelatinase B) was monitored in aorta extracts and in serum-free conditioned cell culture medium obtained from wild-type (WT) mice and from mice with deficiency of tissue-type plasminogen activator (t-PA(-/-)), urokinase-type plasminogen activator (u-PA(-/-)), plasminogen activator inhibitor-1 (PAI-1(-/-)) or plasminogen (Plg(-/-)). In aorta extracts, the contribution of active MMP-2 to the total MMP-2 level ranged between 7 and 16% for the different genotypes, whereas active MMP-9 was not detected. The contribution of active 58 kDa MMP-2 to the total MMP-2 level (active plus latent) ranged between 14 and 29% (mean of 3 experiments) for fibroblasts of the different genotypes, and between 18 and 32% for smooth muscle cells, and was relatively constant in time (7-72 h). The contribution of active 83 kDa MMP-9 to the total MMP-9 level ranged between 15 and 29% for fibroblasts of the different genotypes and was relatively constant in time (24-72 h); corresponding values were 17 to 57% for smooth muscle cells, with the exception...Continue Reading

Citations

Oct 12, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S M Ellerbroek, M S Stack
May 30, 2007·Seminars in Nephrology·Pierre RoncoChristos Chatziantoniou
Jul 29, 2006·Magnetic Resonance Imaging Clinics of North America·Pamela W SchaeferR Gilberto Gonzalez
Dec 20, 2005·Neuroimaging Clinics of North America·Pamela W SchaeferR Gilberto Gonzalez
Nov 22, 2015·Advanced Drug Delivery Reviews·Beate HeissigKoichi Hattori
Jun 23, 2009·Journal of Cellular and Molecular Medicine·Valerie BorelVincent Sapin
May 19, 2012·The Journal of Surgical Research·Yiping ZouMark G Davies
Feb 22, 2012·Biochimica Et Biophysica Acta·Massimiliano CuccioloniMauro Angeletti
Mar 29, 2008·Cell Stem Cell·Beate HeissigKoichi Hattori
Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R C BenyonM J Arthur

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