Regulation of gene expression by the CYP27B1 promoter-study of a transgenic mouse model

The Journal of Steroid Biochemistry and Molecular Biology
Ivanka HendrixH A Morris

Abstract

The enzyme 25-hydroxyvitamin D 1-hydroxylase (CYP27B1) is the rate limiting enzyme in the two-step activation process of Vitamin D to its active form 1,25-dihydroxyvitamin D (1,25D) and is located in the mitochondrial fraction of the proximal tubular cells of the kidney. More recently CYP27B1 activity and expression have also been identified in a number of non-renal cells, which is suggestive of new, previously unidentified roles for Vitamin D in the human body. Although the regulation of CYP27B1 activity and expression has been a major focus of interest over the past decades, the exact molecular mechanism behind the regulation of CYP27B1 activity and expression and the role of the CYP27B1 promoter, herein, are still poorly understood. In this study, we created a transgenic mouse model that expresses the luciferase reporter gene under the control of the full-length, 1.5kb, human CYP27B1 promoter. This animal model allows us to study in vivo the tissue-specific, CYP27B1 promoter-controlled, regulation of the expression of the CYP27B1 gene.

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Citations

Sep 30, 2010·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·Y ZhangH-W Deng
Aug 7, 2008·The Journal of Pharmacology and Experimental Therapeutics·Connie Cheung, Frank J Gonzalez
Sep 11, 2013·PloS One·Ankanee ChanakulFarzana Perwad
May 9, 2014·Frontiers in Physiology·Irfete S FetahuEnikő Kállay
Mar 4, 2008·Molecular and Cellular Endocrinology·Paul H AndersonHoward A Morris
May 3, 2014·Annals of Laboratory Medicine·Howard A Morris
Jun 25, 2017·Scientific Reports·Bin LiLeming Shi
Jan 1, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Kevin N KeaneJohn L Yovich
Jun 16, 2009·Molecular and Cellular Endocrinology·A G TurnerH A Morris
Jul 6, 2011·Molecular and Cellular Endocrinology·Howard A Morris, Paul H Anderson

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