Regulation of Gene Expression during the Onset of Ligninolytic Oxidation by Phanerochaete chrysosporium on Spruce Wood

Applied and Environmental Microbiology
Premsagar KorripallyK E Hammel

Abstract

Since uncertainty remains about how white rot fungi oxidize and degrade lignin in wood, it would be useful to monitor changes in fungal gene expression during the onset of ligninolysis on a natural substrate. We grew Phanerochaete chrysosporium on solid spruce wood and included oxidant-sensing beads bearing the fluorometric dye BODIPY 581/591 in the cultures. Confocal fluorescence microscopy of the beads showed that extracellular oxidation commenced 2 to 3 days after inoculation, coincident with cessation of fungal growth. Whole transcriptome shotgun sequencing (RNA-seq) analyses based on the v.2.2 P. chrysosporium genome identified 356 genes whose transcripts accumulated to relatively high levels at 96 h and were at least four times the levels found at 40 h. Transcripts encoding some lignin peroxidases, manganese peroxidases, and auxiliary enzymes thought to support their activity showed marked apparent upregulation. The data were also consistent with the production of ligninolytic extracellular reactive oxygen species by the action of manganese peroxidase-catalyzed lipid peroxidation, cellobiose dehydrogenase-catalyzed Fe(3+) reduction, and oxidase-catalyzed H2O2 production, but the data do not support a role for iron-chelati...Continue Reading

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Citations

Sep 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jiwei ZhangJonathan S Schilling
Feb 21, 2018·The Journal of Biological Chemistry·Carl J HoutmanKenneth E Hammel
Jun 5, 2016·Applied and Environmental Microbiology·Marianne DaouCraig B Faulds
Sep 14, 2018·Environmental Microbiology·Antonio José Fernández-GonzálezMélanie Morel-Rouhier
Nov 9, 2018·Frontiers in Bioengineering and Biotechnology·Gustavo Pagotto BorinJuliana Velasco de Castro Oliveira
Sep 28, 2018·Microbiology and Molecular Biology Reviews : MMBR·Bastien BissaroVincent G H Eijsink
Nov 4, 2020·Environmental Microbiology·Aurin M VosHan A B Wösten
Feb 5, 2021·Environmental Microbiology·Hayat HageMarie-Noëlle Rosso
Dec 12, 2018·Fungal Genetics and Biology : FG & B·Jiwei ZhangJonathan Schilling
May 29, 2021·Carbohydrate Research·Tamilvendan ManavalanVincent G H Eijsink

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