PMID: 6164326Jan 1, 1980Paper

Regulation of gene expression in primary cultures of adult rat hepatocytes on collagen gels

Annals of the New York Academy of Sciences
J T SpenceH C Pitot

Abstract

The studies described in this paper demonstrate rather conclusively the efficacy of the study of the regulation of gene expression in primary cultures of adult rat hepatocytes. The utilization of these cells in completely defined medium allows one to determine the exact environmental conditions for the regulation of the expression of specific genes. In the studies described in this work, we have demonstrated that the regulation of glucokinase involved three hormones, insulin, corticosteroids, and T3. In contrast, the regulation of an enzyme involved primarily in fatty acid metabolism, ATP-citrate lyase, required only insulin and T3 for its full expression. Cyclic GMP appeared to be involved in the regulation of glucokinase, but not ATP-citrate lyase, a fact that would be extremely difficult to demonstrate clearly in vivo. The regulation of the gluconeogenic enzyme, ornithine aminotransferase, in vitro involved only a single hormone, glucagon, the inhibition of induction by corticoid steroids demonstrable in vivo being absent in cell culture. However, the repressive effect of glucose on the induction of this enzyme was quite comparable to that seen in vivo and was not mediated through cyclic AMP or insulin, based on findings in ...Continue Reading

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A E SiricaH C Pitot
Feb 3, 1975·European Journal of Biochemistry·N C PartridgeI T Oliver
Apr 30, 1977·Archives of Biochemistry and Biophysics·R T Lyons, H C Pitot
May 1, 1977·Nutrition Reviews·G Wolf
Jul 3, 1978·Life Sciences·M G Cherian, R A Goyer
Sep 26, 1966·Biochimica Et Biophysica Acta·A K RoyC R Harmison
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·G IllianoP Cuatrecasas
Jul 28, 1968·Journal of Molecular Biology·D K GrannerG M Tomkins
Jan 1, 1973·Physiological Reviews·H C Pitot, M B Yatvin
May 7, 1974·Biochemical and Biophysical Research Communications·S C Kuo, J O Lampen
Jan 1, 1972·Advances in Enzyme Regulation·D M GibsonY Muto
Jan 1, 1969·Annual Review of Pharmacology·R Kuntzman
Jan 1, 1964·Advances in Enzyme Regulation·H C PitotA L Kennan
Dec 21, 1965·Biochemical and Biophysical Research Communications·J Brown, P McLean
Dec 1, 1964·Analytical Biochemistry·H C PITOT, N PRIES
Oct 9, 1964·Biochimica Et Biophysica Acta·S ABRAHAMI L CHAIKOFF

❮ Previous
Next ❯

Citations

Sep 1, 1983·The Journal of Cell Biology·H Baumann, G P Jahreis
Apr 16, 2003·Human & Experimental Toxicology·Margarita KataropoulouHelen Grant
Oct 1, 1982·European Journal of Biochemistry·W SibrowskiH J Seitz
Mar 1, 1985·Archives of Biochemistry and Biophysics·M J Merrill, H C Pitot
May 15, 1981·Biochemical and Biophysical Research Communications·C NodaA Ichihara

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.