Regulation of gene expression of epithelial calcium channels in intestine and kidney of mice by 1alpha,25-dihydroxyvitamin D3

The Journal of Steroid Biochemistry and Molecular Biology
Toshio OkanoS Kato

Abstract

In wild-type (VDR(+/+)) mice, ECaC2 expression was confirmed in the intestine and kidney, while ECaC1 expression was exclusively confined to the kidney. Both mRNAs expression of ECaC1 and ECaC2 in the kidney and ECaC2 mRNA expression in the intestine increased time- and dose-dependently in response to 1alpha,25(OH)(2)D(3) injection in VDR(+/+) mice, but not in VDR(-/-) mice. The mRNA levels of ECaC2 in the intestine of VDR(-/-) mice were remarkably reduced when compared to VDR(+/+) mice, while no significant differences were observed in both mRNA levels of ECaC1 and ECaC2 in the kidney between VDR(+/+) mice and VDR(-/-) mice. In the primary renal tubular cells (PRTC) isolated from VDR(+/+) mice, both ECaCs mRNA expression increased in response to 1alpha,25(OH)(2)D(3) treatment, but not in the PRTC of VDR(-/-) mice. PTH increased both ECaCs mRNA expression in the PRTC of VDR(+/+) mice. These results suggest that 1alpha,25(OH)(2)D(3) directly modulates the gene expression of ECaC1 and ECaC2 together with PTH in the kidney of mice. 1alpha,25(OH)(2)D(3) also modulates the gene expression of ECaC2 in the intestine of mice, however, further studies are needed to elucidate the direct action of 1alpha,25(OH)(2)D(3) on the expression of...Continue Reading

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Citations

Sep 5, 2007·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·Y ZhangM-S Wong
Jun 1, 2005·Pflügers Archiv : European journal of physiology·Els den DekkerRené J M Bindels
Sep 13, 2008·Clinical and Experimental Pharmacology & Physiology·Ramesh C Khanal, Ilka Nemere
Sep 29, 2011·Cellular and Molecular Life Sciences : CMLS·Sandor BorosJoost G Hoenderop
Dec 5, 2008·Nutrition Reviews·Daniel R Schmidt, David J Mangelsdorf
Jul 5, 2008·Annual Review of Nutrition·Ramesh C Khanal, Ilka Nemere
Dec 5, 2016·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·M R WilkensA Cehak
Feb 28, 2018·The Journal of Physiological Sciences : JPS·Kornkamon LertsuwanNarattaphol Charoenphandhu
Sep 15, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yingben XueDengshun Miao
Feb 27, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Claudia PalmKarin Weber
Jan 18, 2014·The EMBO Journal·Olena AndrukhovaReinhold G Erben
Aug 11, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Titia E Woudenberg-VrenkenJoost G J Hoenderop

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