Regulation of glucose carriers in chick fibroblasts

Journal of Supramolecular Structure
H AmosH Asdourian

Abstract

The derepression of glucose transport initiated by removing glucose from the incubation medium requires both protein and RNA synthesis. The synthesis and accumulation of putative mRNA for the carrier protein(s) can be demonstrated by inhibiting protein synthesis with cycloheximide (2 microgram/ml). Release from inhibition with simultaneous addition of actinomycin D (1-5 microgram/ml) results in a burst of carrier synthesis that achieves virtually maximal derepression in 4-6 h. An external energy source provided by a "nonrepressive" sugar (D-fructose, D-xylose) or by pyruvate is required to accomplish carrier synthesis. Previous failure to demonstrate mRNA accumulation was due to the depletion of energy in the starved cells. Glucose acts as a repressor at a posttranscriptional step, probably at the level of turnover of formed carrier. The protection of formed carrier in the absence of glucose and by inhibitors of protein synthesis even in the presence of glucose has encouraged conjecture that a protease is activated by a metabolic product of glucose that is analogous to a corepressor. The glucose metabolite either activates the protease by direct interaction with it or alters the conformation of the carrier to expose a critical ...Continue Reading

References

Nov 1, 1976·Journal of Cellular Physiology·W A PeckM A Lichtman
Apr 1, 1975·Archives of Biochemistry and Biophysics·D UllreyH M Kalckar
May 1, 1977·Journal of Cellular Physiology·T A MuslinerH Amos
Dec 1, 1976·Journal of Cellular Physiology·D L Oxender, S C Quay
Dec 1, 1976·Journal of Cellular Physiology·H AmosT A Musliner
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·C W ChristopherM Kalckar
Jun 1, 1975·Archives of Biochemistry and Biophysics·P RossowH Amos
Jan 1, 1966·Cold Spring Harbor Symposia on Quantitative Biology·G M TomkinsB Peterkofsky
Apr 29, 1974·Biochimica Et Biophysica Acta·M Hatanaka
Jul 28, 1968·Journal of Molecular Biology·D K GrannerG M Tomkins
Nov 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·R MartineauH Amos
May 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M Hatanaka
Sep 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·H M Kalckar, D Ullrey
Jul 2, 1973·Biochemical and Biophysical Research Communications·S N Shaw, H Amos
Jun 1, 1949·Proceedings of the Society for Experimental Biology and Medicine·J H HANKS, R E WALLACE

❮ Previous
Next ❯

Citations

Oct 1, 1993·Baillière's Clinical Endocrinology and Metabolism·W T Garvey, M J Birnbaum
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C W Christopher, R A Morgan
Sep 1, 1982·Journal of Cellular Physiology·R J GerminarioM Taylor
Apr 1, 1982·Journal of Cellular Physiology·A H Romano, N D Connell
Feb 13, 1986·Biochemical and Biophysical Research Communications·K P FungC Y Lee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.