Regulation of glucose transport and c-fos and egr-1 expression in cells with mutated or endogenous growth hormone receptors

Endocrinology
T W GongJ Schwartz

Abstract

To identify mechanisms by which GH receptors (GHR) mediate downstream events representative of growth and metabolic responses to GH, stimulation by GH of c-fos and egr-1 expression and glucose transport activity were examined in Chinese hamster ovary (CHO) cells expressing mutated GHR. In CHO cells expressing wild-type GHR(GHR(1-638)), GH stimulated the expression of c-fos and egr-1, and stimulated 2-deoxyglucose uptake, responses also mediated by endogenous GHR in 3T3-F442A cells. Deletion of the proline-rich box 1 of GHR (GHR(deltaP)) abrogated all of these responses to GH, indicating that box 1, a site of association of GHR with the tyrosine kinase JAK2, is crucial for these GH-stimulated responses. As the C-terminal half of the cytoplasmic domain of GHR is required for GH-stimulated calcium flux and for stimulation of spi-2.1 transcription, GHR lacking this sequence (GHR(1-454)) were examined. Not only did GHR(1-454) mediate stimulation of c-fos and egr-1 expression and 2-deoxyglucose uptake, but they also mediated GH-stimulated transcriptional activation via Elk-1, a transcription factor associated with the c-fos Serum Response Element. Thus, the C-terminal half of the cytoplasmic domain of GHR is not required for GH-stimu...Continue Reading

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Citations

Feb 5, 2011·Molecular Endocrinology·Tracy X CuiJessica Schwartz
Mar 13, 2012·The Journal of Membrane Biology·Manzar ShojaiefardFlorian Lang
Feb 25, 2014·The Journal of Membrane Biology·Zohreh HosseinzadehFlorian Lang
Feb 28, 2002·Endocrinology·Váronique BeauloyeJean-Paul Thissen
Jan 20, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yoshiaki ItoigawaEiji Itoi
Apr 27, 2002·Scandinavian Journal of Immunology·W SavinoM Dardenne
Apr 4, 2014·American Journal of Physiology. Cell Physiology·Zohreh HosseinzadehFlorian Lang
May 18, 1999·The Journal of Biological Chemistry·H YamashitaH Rui
Feb 21, 2003·Biochemical and Biophysical Research Communications·Christina N BennettJessica Schwartz

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