Regulation of glucose transport in the NIH 3T3 L1 preadipocyte cell line by TCDD

Environmental Health Perspectives
H OlsenF Matsumura

Abstract

This study examined the changes in cellular glucose uptake induced by 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) as measured by quantification of intracellular radioactivity in the NIH 3T3 L1 preadipocyte cell line after a 30-minute incubation with the non-metabolizable radioactive analogue of glucose, 3-O-methyl-D-[1-3H] glucose. Treatment of differentiated NIH 3T3 L1 cells with TCDD produced a time- and dose-dependent decrease in the cellular uptake of glucose. Treatment of cells for 3 hr with 10(-8) M TCDD significantly reduced glucose uptake to about 10% of control values (p </= 0.05). Furthermore, cytochalasin B, a specific inhibitor of facilitative glucose transporter proteins totally abolished the portion of glucose transport activity that is sensitive to TCDD. The role of the Ah receptor in TCDD-mediated reduction in glucose uptake was investigated. Pretreatment of 3T3 L1 cells with the Ah receptor blocker 4,7-phenanthroline antagonized the effect of TCDD on glucose uptake. Structure-activity relationship studies with TCDD and two polychlorinated biphenyl (PCB) congeners revealed a rank order for their potency in the inhibition of glucose transport as follows: TCDD <3,3',4,4' tetrachlorobiphenyl <2,2',5,5' tetrachlorobi...Continue Reading

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Citations

May 7, 2002·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Hitoshi Nagashima, Fumio Matsumura
Oct 29, 2014·Journal of Toxicology·Dinesh Babu JestadiLatha Periyasamy
Nov 17, 2006·Journal of Epidemiology and Community Health·Duk-Hee LeeMiquel Porta
Oct 20, 2009·PloS One·Anna Rignell-HydbomLars Rylander
Aug 8, 2001·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·F El-SabeawyB Lasley
Jul 12, 2007·Human & Experimental Toxicology·Anna Rignell-HydbomLars Hagmar
May 8, 2014·Reproductive Sciences·Kenan OmurtagKelle H Moley
Apr 15, 2006·Journal of Biochemical and Molecular Toxicology·Phillip Chin-Chen Liu, Fumio Matsumura
Feb 6, 2019·International Journal of Environmental Research and Public Health·Yeon A KimYoung Hyun Yoo
Jun 19, 2008·Reviews on Environmental Health·David O Carpenter
Apr 23, 2019·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Eun Mi ChoiSuk Chon
Dec 4, 2010·Journal of Environmental Monitoring : JEM·Charles Jay EverettMarty Player
Oct 3, 2003·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·V BruggemanE Decuypere
Dec 8, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ho Jae HanJang Hern Lee

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