PMID: 6113586Jan 1, 1981Paper

Regulation of glutamine synthetase activity by adenylylation in the Gram-positive bacterium Streptomyces cattleya

Proceedings of the National Academy of Sciences of the United States of America
S L Streicher, B Tyler

Abstract

The enzymatic activity of glutamine synthetase [GS; L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2] from the Gram-positive bacterium Streptomyces cattleya is regulated by covalent modification. In whole cells containing high levels of GS the addition of ammonium chloride leads to a rapid decline in GS activity. Crude extracts prepared from such ammonia-shocked cells had very low levels of GS activity as measured by biosynthetic and gamma-glutamyltransferase assays. Incubation of the crude extracts with snake venom phosphodiesterase restored GS activity. In cell extracts, GS was also inactivated by an ATP- and glutamine-dependent reaction. Radioactive labeling studies demonstrated the incorporation of an AmP moiety into GS protein upon modification. Our results suggest a covalent modification of GS in a Gram-positive bacterium. This modification appears to be adenylylation of the GS subunit similar to that found in the Gram-negative bacteria.

References

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Apr 3, 1968·European Journal of Biochemistry·C Gancedo, H Holzer

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Citations

Sep 1, 1995·Current Microbiology·C Palaniappan, M Gunasekaran
Jan 1, 1984·Molecular & General Genetics : MGG·S KustuJ C Meeks
Dec 1, 1984·Gene·S H FisherA L Sonenshein
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Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J EdmandsD R Benson
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G W McCarty, J M Bremner
Jan 1, 1988·Antonie van Leeuwenhoek·N IllingD R Woods
Sep 30, 1988·Biochemical and Biophysical Research Communications·K KimuraY Nakano
Nov 23, 2006·Journal of Bioscience and Bioengineering·Masafumi KameyaYasuo Igarashi
Apr 1, 1990·Molecular & General Genetics : MGG·A M SanangelantoniO Tiboni
May 1, 1989·Journal of Bacteriology·S H Fisher, L V Wray
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Jan 1, 1985·Journal of Bacteriology·S NordlundP W Ludden
Jul 1, 1983·Journal of Bacteriology·K E Kendrick, J C Ensign
Apr 1, 1986·Journal of Bacteriology·N A Noridge, D R Benson

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