PMID: 6108785Jun 13, 1980Paper

Regulation of glutamine synthetase in the blue-green alga Anabaena L-31

Biochimica Et Biophysica Acta
R Tuli, J Thomas

Abstract

In N2-grown cultures of Anabaena L-31, in which protein synthesis was prevented by chloramphenicol, presence of NH+4 caused a drastic decrease of glutamine synthetase (L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2) activity indicating NH+4-mediated inactivation or degradation of the enzyme. The half-life of glutamine synthetase was more than 24 h, whereas that of nitrogenase (reduced ferredoxin:dinitrogen oxidoreductase (ATP-hydrolysing), EC 1.18.2.1) was less than 4 h, suggesting that glutamine synthetase may not act as positive regulator of nitrogenase synthesis in Anabaena. Glutamine synthetase purified to homogeneity was subject to cumulative inhibition by alanine, serine and glycine. The amino acids, however, exhibited partial antagonism in this behaviour. Glyoxylate, an intermediate in photorespiration, virtually prevented the amino acid inhibition. Kinetic studies revealed inhibition of the enzyme activity by high Mg2+ concentration under limiting glutamate level and by high glutamate in limiting Mg2+. Maximum enzyme activity occurred when the ratio of glutamate to free Mg2+ was 0.5 to 1.0. The results demonstrate that the enzyme is subject to multiple regulation by various metabolites involved in nitrogen assimil...Continue Reading

References

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Citations

Jan 1, 1981·Archives of Biochemistry and Biophysics·R Tuli, J Thomas
Sep 1, 1991·JPEN. Journal of Parenteral and Enteral Nutrition·M Wusteman, M Elia
Aug 27, 2019·Angewandte Chemie·Iker LeónJose Luis Alonso
Oct 1, 1985·Applied and Environmental Microbiology·J A Patterson, R B Hespell

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