Regulation of glycogen utilization, but not glucose utilization, by precontraction glycogen levels in vascular smooth muscle

Biochemistry
C D Hardin, T M Roberts

Abstract

These experiments were designed to determine whether glycogenolysis was influenced by the glycogen concentration of vascular smooth muscle. Segments of hog carotid artery smooth muscle were allowed to synthesize variable amounts of 1-[13C]glucosyl units of glycogen. Artery segments were then isometrically contracted in the presence of 2-[13C]glucose. Prior to and after isometric contraction, measurements were made of tissue glycogen content and superfusate glucose and lactate concentrations. 2-[13C]Lactate and 3-[13C]lactate peak intensities in the superfusate were measured using 13C-NMR spectroscopy. The tissue glycogen content decreased exponentially during the 4.5 h of isometric contraction (R2 = 0.990), despite more than a 3-fold range of glycogen concentration prior to contraction. The extent of glycogen utilization during a 3 h isometric contraction varied linearly with the precontraction glycogen concentration (R2 = 0.727). Lactate production specifically from glycogen breakdown increased with an increase in precontraction glycogen concentration (R2 = 0.620). During a 3 h isometric contraction neither the glucose utilization (R2 = 0.007) nor lactate production specifically produced from glucose (R2 = 0.00002) varied with...Continue Reading

References

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Citations

Jun 8, 2012·The Journal of Veterinary Medical Science·Takeharu KanedaKazumasa Shimizu
Aug 9, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Jia ShiFan He
Jun 14, 2000·American Journal of Physiology. Heart and Circulatory Physiology·T J Allen, C D Hardin
Feb 7, 2001·American Journal of Physiology. Cell Physiology·P G Lloyd, C D Hardin
Dec 22, 1999·The American Journal of Physiology·P G Lloyd, C D Hardin
Feb 12, 1998·The American Journal of Physiology·C D Hardin, D R Finder
Dec 9, 1998·The American Journal of Physiology·T M Allen, C D Hardin

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