Regulation of G(q/11)alpha by the gonadotropin-releasing hormone receptor

Molecular Endocrinology
D StanislausP M Conn

Abstract

Evidence from use of pertussis and cholera toxins and from NaF suggested the involvement of G proteins in GnRH regulation of gonadotrope function. We have used three different methods to assess GnRH receptor regulation of G(q/11)alpha subunits (G(q/11)alpha). First, we used GnRH-stimulated palmitoylation of G(q/11)alpha to identify their involvement in GnRH receptor-mediated signal transduction. Dispersed rat pituitary cell cultures were labeled with [9,10-(3)H(N)]-palmitic acid and immunoprecipitated with rabbit polyclonal antiserum made against the C-terminal sequence of G(q/11)alpha. The immunoprecipitates were resolved by 10% SDS-PAGE and quantified. Treatment with GnRH resulted in time-dependent (0-120 min) labeling of G(q/11)alpha. GnRH (10(-12), 10(-10), 10(-8), or 10(-6) g/ml) for 40 min resulted in dose-dependent labeling of G(q/11)alpha compared with controls. Cholera toxin (5 microg/ml; activator of G(i)alpha), pertussis toxin (100 ng/ml; inhibitor of G(i)alpha actions) and Antide (50 nM; GnRH antagonist) did not stimulate palmitoylation of G(q/11)alpha above basal levels. However, phorbol myristic acid (100 ng/ml; protein kinase C activator) stimulated the palmitoylation of G(q/11)alpha above basal levels, but not t...Continue Reading

References

Jul 1, 1992·Molecular Endocrinology·M TsutsumiS C Sealfon
Jul 1, 1994·Cell·P B Wedegaertner, H R Bourne
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·S M MumbyA G Gilman

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Citations

Sep 15, 2010·Brain Research·Amy M NavratilMark S Roberson
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·C HuangS M Mumby
May 3, 2012·The Journal of Biological Chemistry·Soon-Gang ChoiStuart C Sealfon
Jun 24, 2010·American Journal of Physiology. Endocrinology and Metabolism·Peter Y LiuRonald S Swerdloff
Jan 25, 2008·Journal of Neurophysiology·Christopher P FordPeter A Smith
Apr 21, 2001·Oncogene·C A Chen, D R Manning
Feb 23, 2021·Frontiers in Endocrinology·Colin M ClayAmy M Navratil

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