Abstract
Cellular levels of phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) are rapidly elevated in response to activation of growth factor receptor tyrosine kinases. This polyphosphoinositide binds the pleckstrin homology (PH) domain of GRP1, a protein that also contains 200 residues with high sequence similarity to a segment of the yeast Sec7 protein that functions as an ADP ribosylation exchange factor (ARF) (Klarlund, J., Guilherme, A., Holik, J. J., Virbasius, J. V., Chawla, A., and Czech, M. P. (1997) Science 275, 1927-1930). Here we show that dioctanoyl PtdIns(3,4,5)P3 binds the PH domain of GRP1 with a Kd = 0.5 microM, an affinity 2 orders of magnitude greater than dioctanoyl-PtdIns(4,5)P2. Further, the Sec7 domain of GRP1 is found to catalyze guanine nucleotide exchange of ARF1 and -5 but not ARF6. Importantly, PtdIns(3,4,5)P3, but not PtdIns(4,5)P2, markedly enhances the ARF exchange activity of GRP1 in a reaction mixture containing dimyristoylphosphatidylcholine micelles, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid, and a low concentration of sodium cholate. PtdIns(3,4,5)P3-mediated ARF nucleotide exchange through GRP1 is selectively blocked by 100 microM inositol 1,3,4,5-tetrakisphosphate, which ...Continue Reading
References
May 26, 1995·The Journal of Biological Chemistry·J Moss, M Vaughan
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·S C TsaiM Vaughan
Oct 7, 1993·Biochimica Et Biophysica Acta·L R StephensP T Hawkins
Jan 19, 1996·The Journal of Biological Chemistry·M FrancoS Paris
Aug 1, 1995·Cellular Signalling·S NakanishiY Matsuda
Mar 15, 1996·Science·P De CamilliP Novick
May 31, 1996·Cell·M A LemmonJ Schlessinger
Feb 1, 1996·The Journal of Cell Biology·H ShpetnerS Corvera
Feb 29, 1996·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M J ZvelebilF Pages
Aug 8, 1996·Biochimica Et Biophysica Acta·C L Carpenter, L C Cantley
Apr 1, 1996·Current Opinion in Cell Biology·C L Carpenter, L C Cantley
Jun 1, 1996·Current Opinion in Immunology·R T Abraham
Oct 11, 1996·The Journal of Biological Chemistry·A PtasznikG M Bokoch
Dec 5, 1996·Nature·A PeyrocheC L Jackson
Dec 5, 1996·Nature·P ChardinM Chabre
Jan 1, 1996·Annual Review of Cell and Developmental Biology·I Mellman
Dec 1, 1996·Journal of Molecular Endocrinology·P R ShepherdS O'Rahilly
Feb 14, 1997·The Journal of Biological Chemistry·J O Liang, S Kornfeld
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·E MeacciM Vaughan
Mar 28, 1997·Science·J K KlarlundM P Czech
Apr 1, 1997·Current Biology : CB·F SchimmöllerS Pfeffer
Jun 12, 1997·Nature·A Toker, L C Cantley
Jul 1, 1997·Trends in Biochemical Sciences·B VanhaesebroeckM D Waterfield
Aug 29, 1997·The Journal of Biological Chemistry·L E RamehL C Cantley
Mar 1, 1996·Trends in Cell Biology·P R ShepherdH W Davidson
Citations
Feb 27, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C A Baumann, A R Saltiel
Jan 25, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·Paschal A Oude WeerninkMartina Schmidt
Jul 3, 2007·Pflügers Archiv : European journal of physiology·He-Ping MaDouglas C Eaton
Jun 8, 1999·Chemistry and Physics of Lipids·M G RothS Yu
Feb 28, 2001·Journal of Immunological Methods·V GarceauS G Bourgoin
Oct 26, 1999·Cellular Signalling·G Berton, C A Lowell
Apr 21, 1999·Current Opinion in Cell Biology·S J LeeversM D Waterfield
Feb 19, 2002·Trends in Cell Biology·Alan R Saltiel, Jeffrey E Pessin
Dec 17, 1998·Trends in Cell Biology·S Corvera, M P Czech
May 14, 1999·Trends in Cell Biology·M G Roth
Feb 1, 2000·Trends in Cell Biology·C L Jackson, J E Casanova
Oct 26, 2000·Trends in Biochemical Sciences·T R JacksonA B Theibert
Nov 5, 1999·Trends in Biochemical Sciences·N BlombergM Saraste
Sep 13, 2000·Molecular Cell·S E LietzkeD G Lambright
Jul 30, 1999·Biochimica Et Biophysica Acta·D JonesS Cockcroft
Dec 22, 2004·Biochemistry·John A CorbinJoseph J Falke
Mar 24, 2006·Nature Methods·Tor Erik Rusten, Harald Stenmark
Dec 20, 2011·Oncogene·B MiaoA Degterev
Nov 24, 1999·The Biochemical Journal·P B OateyJ M Tavaré
Dec 10, 1999·The Biochemical Journal·A GrayC P Downes
Jul 6, 2000·The Biochemical Journal·S DowlerD R Alessi
Aug 6, 2000·The Biochemical Journal·M A Lemmon, K M Ferguson
Sep 26, 2000·The Biochemical Journal·S DowlerD R Alessi
Feb 15, 2001·The Biochemical Journal·S StratfordS A Summers
Jun 10, 2000·European Journal of Biochemistry·T KnorrW Kolanus
Oct 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Youxin ZhangJoel A Swanson
Aug 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Olof Idevall-HagrenPietro De Camilli
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·S F BetzS W Fesik
Nov 5, 1998·Molecular Biology of the Cell·S R FrankJ E Casanova
Jan 27, 2004·Molecular Biology of the Cell·Randal CoxNava Segev
Apr 6, 2007·Molecular Biology of the Cell·Lee Ann CohenJulie G Donaldson
Jul 3, 1998·The EMBO Journal·S Béraud-DufourB Antonny
Sep 16, 1998·The EMBO Journal·S J IsakoffE Y Skolnik
Mar 17, 1999·The EMBO Journal·M FrancoP Chavrier
Jan 29, 2000·Molecular and Cellular Biology·G A RodriguesJ Schlessinger
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·K A Gottlieb, L P Villarreal
Jan 19, 1999·Annual Review of Cell and Developmental Biology·T F Martin
Jul 7, 2014·BMC Research Notes·Yi-An ChenKenji Mizuguchi
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·A SomeyaM Vaughan
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·M SataM Vaughan
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·M FrancoP Chavrier
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·P A RandazzoJ A Cooper
Dec 18, 2009·Molecular Biology of the Cell·David T WhiteLorraine C Santy
Apr 4, 2007·The Journal of Cell Biology·Arlinet KierbelJoanne Engel
Oct 24, 1998·The Journal of Cell Biology·P Várnai, T Balla
Nov 4, 2000·The Journal of Cell Biology·T R JacksonP A Randazzo
Mar 16, 2016·The Journal of Physical Chemistry Letters·Fiona B NaughtonMark S P Sansom
Oct 7, 2004·European Journal of Pharmacology·Paschal A Oude WeerninkKarl H Jakobs
Apr 25, 2000·Experimental Cell Research·S Y LeeB Pohajdak
Jan 9, 2003·Annual Review of Physiology·Michael P Czech