PMID: 7580871Aug 1, 1995Paper

Regulation of GTP cyclohydrolase I gene expression and tetrahydrobiopterin content by nerve growth factor in cultures of superior cervical ganglia

Neurochemistry International
K Hirayama, Gregory Kapatos

Abstract

Monolayer cultures of superior cervical ganglia free of support cells were maintained in the presence of 100 ng/ml 7S-NGF for 4 days. The concentration of NGF was then changed to between 50 and 400 ng/ml and cultures continued for an additional 7 days. Tetrahydrobiopterin (BH4) content, GTP cyclohydrolase (GTPCH) enzyme activity and mRNA levels were then determined. All three of these measures were found to be elevated between 2- to 4-fold by treatment with increasing concentrations of NGF. Tyrosine hydroxylase (TH) enzyme activity and mRNA levels were increased from 8 to 13-fold by these same treatments. These results indicate that the content of BH4 within sympathetic neurons can be regulated by NGF receptor-mediated changes in GTPCH gene expression. Moreover, concomitant increases in TH enzyme activity and BH4 content demonstrate a coordinated regulation by NGF of this enzyme and its essential cofactor.

References

May 31, 1988·Biochemical and Biophysical Research Communications·H SuzukiS Yamada
Jan 1, 1985·Annual Review of Biochemistry·C A NicholD S Duch
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M M Abou-Donia, O H Viveros
Feb 1, 1980·Analytical Biochemistry·T Fukushima, J C Nixon

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Citations

Nov 22, 2001·Biochemical and Biophysical Research Communications·J TanakaY Kato
Oct 23, 1997·Brain Research. Molecular Brain Research·L SerovaE L Sabban
Oct 26, 1999·The Journal of Biological Chemistry·T Scherer-OppligerB Thöny

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