Regulation of heat shock protein synthesis by gonadotropins in cultured granulosa cells

Endocrinology
A Ben-Ze'ev, A Amsterdam

Abstract

Primary cultures of granulosa cells can be stimulated to produce large amounts of progesterone by gonadotropins. This stimulation is associated with significant changes in the expression of several major proteins, as revealed by two-dimensional gel electrophoresis. These changes include a decrease in the synthesis of actin cytoskeleton proteins and an increase in the synthesis of a few abundant proteins, one of which is a mammalian heat shock protein, hsp90. Under culture conditions that have previously been shown to bring about the maturation of granulosa cells into progesterone-producing cells (i.e. treatment with gonadotropins or cAMP or by disrupting the actin cytoskeleton with cytochalasin), an increased synthesis of hsp90 could be demonstrated. Freshly isolated granulosa cells isolated from PMSG-treated animals synthesize hsp90 at a much higher level than cells isolated from diethylstilbestrol-treated rats. Kinetic studies have shown that granulosa cells isolated from diethylstilbestrol- or PMSG-treated rats synthesize high levels of hsp90 if maintained in culture in the presence of gonadotropins, but rapidly decrease hsp90 synthesis in the absence of gonadotropins and increase the synthesis of actin cytoskeleton proteins...Continue Reading

Citations

Sep 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·J A McGuireJ A Gustafsson

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