Regulation of histone H4 acetylation by transcription factor E2A in Ig gene conversion

International Immunology
Hiroyuki KitaoMinoru Takata

Abstract

Recent studies implicate the transcription factor E2A in Ig diversification such as somatic hypermutation or gene conversion (GCV). GCV also requires active Ig transcription, expression of the activation-induced deaminase (AID) and a set of homologous recombination factors. We have disrupted the E2A gene in the chicken B-cell line DT40 and found greatly diminished rate of GCV without changes in the levels of transcripts from AID and Ig heavy chain or Ig light chain (IgL) genes. However, chromatin immunoprecipitation analysis revealed that the loss of E2A accompanies drastically reduced acetylation levels of the histone H4 in rearranged IgL locus. Furthermore, the defects in GCV were restored by trichostatin A treatment, which raised H4 acetylation to the normal levels. Thus, E2A may contribute to GCV by maintaining histone acetylation, which could be a prerequisite for targeting or full deaminase function of AID.

References

Oct 10, 2001·BMC Biotechnology·H ArakawaJ M Buerstedde
Mar 8, 2002·Current Biology : CB·Reuben S HarrisMichael S Neuberger
Jun 18, 2002·Science·Kiyotsugu YoshikawaTasuku Honjo
Nov 19, 2002·The Journal of Biological Chemistry·Curtis BradneyYuan Zhuang
Apr 30, 2003·Nature Immunology·Camil Elie SayeghCornelis Murre
Oct 29, 2003·The Journal of Experimental Medicine·Hiroyuki GondaAkira Shimizu
Mar 3, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hiroshi Arakawa, Jean-Marie Buerstedde
Jun 1, 2005·Nature Biotechnology·Hidetaka SeoKunihiro Ohta
Nov 16, 2005·Annual Review of Genetics·Nancy Maizels
Dec 1, 2005·Journal of Cell Science·Sabine M GörischKarsten Rippe
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ulrike SchoetzJean-Marie Buerstedde
Dec 21, 2006·The Journal of Experimental Medicine·Shu Yuan YangDavid G Schatz
Mar 14, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Masami NakayamaTatsuo Nakayama
Jun 20, 2007·Annual Review of Genetics·Grace Teng, F Nina Papavasiliou
Jun 22, 2007·Immunity·Stephen L Nutt, Barbara L Kee
Jul 13, 2007·Sub-cellular Biochemistry·Jean-Marie Buerstedde, Hiroshi Arakawa
Sep 21, 2007·PLoS Biology·W Jason CummingsNancy Maizels

❮ Previous
Next ❯

Citations

May 7, 2013·Trends in Immunology·Guideng LiPaolo Casali
Nov 15, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Hiroshi Arakawa, Jean-Marie Buerstedde
Apr 3, 2014·PLoS Biology·Jean-Marie BuersteddeDavid G Schatz
Jul 28, 2010·PloS One·Glen M BorchertErik D Larson
Jan 27, 2010·The Journal of Experimental Medicine·Atsushi TanakaUrsula Storb
Dec 24, 2015·Frontiers in Immunology·Hong Zan, Paolo Casali
Aug 25, 2009·Molecular Immunology·Yonghwan Kim, Ming Tian
May 25, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hiroyuki KitaoMinoru Takata
Jan 1, 2011·Genes·Kohei Kurosawa, Kunihiro Ohta
Dec 26, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Munehisa YabukiNancy Maizels
Oct 23, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kristin M KohlerDavid G Schatz
Jul 10, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jessica J McDonaldDavid G Schatz
Jan 10, 2021·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Natsuki TakamuraKunihiro Ohta

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.