Regulation of Human Airway Epithelial Tissue Stem Cell Differentiation by β-Catenin, P300, and CBP

Stem Cells
Daniel T MalleskeSusan D Reynolds

Abstract

The wingless/integrase-1 (WNT)/β-catenin signaling pathway is active in several chronic lung diseases including idiopathic pulmonary fibrosis, asthma, and chronic obstructive pulmonary disease. Although this WNT/β-catenin pathway activity is associated with an increase in mucus cell frequency and a decrease in ciliated cell frequency, a cause and consequence relationship between signaling and cell frequency has not been established. We previously demonstrated that genetic stabilization of β-catenin inhibited differentiation of mouse bronchiolar tissue stem cells (TSC). This study determined the effect of β-catenin and its co-factors P300 (E1A-binding protein, 300 kDa) and cAMP response element binding (CREB)-binding protein (CBP) on human bronchial epithelial TSC differentiation to mucus and ciliated cells. We developed a modified air-liquid interface (ALI) culture system in which mucus and ciliated cell frequency is similar. These cultures were treated with the β-catenin agonist CHIR99021 (CHIR) and antagonists to β-catenin (XAV939), P300 (IQ1), and CBP (ICG001). We report that human TSC differentiation to mucus and ciliated cells can be divided into two stages, specification and commitment. CHIR treatment inhibited mucus and ...Continue Reading

References

Feb 1, 1991·American Journal of Respiratory Cell and Molecular Biology·C H ChichesterC G Plopper
Jun 1, 1994·American Journal of Respiratory Cell and Molecular Biology·R R MercerJ D Crapo
Jul 13, 2000·Current Opinion in Genetics & Development·E V Rothenberg
Dec 6, 2001·American Journal of Respiratory and Critical Care Medicine·P K Jeffery
Oct 22, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yingjian YouSteven L Brody
Feb 13, 2003·Nucleic Acids Research·Rafael A IrizarryTerence P Speed
Apr 23, 2003·The American Journal of Pathology·Marco ChilosiClaudio Doglioni
Jun 24, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yingjian YouSteven L Brody
Jul 30, 2003·The Journal of Biological Chemistry·Michael L MucenskiJeffrey A Whitsett
Aug 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Katayoon H EmamiMichael Kahn
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
Jul 26, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Michael L MucenskiJeffrey A Whitsett
Aug 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jia-Ling TeoMichael Kahn
Jun 24, 2006·The Journal of Biological Chemistry·Michael D Gordon, Roel Nusse
Mar 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tomoyuki MiyabayashiMichael Kahn
Mar 22, 2008·Stem Cells·Susan D ReynoldsBarry R Stripp
Feb 14, 2009·American Journal of Respiratory Cell and Molecular Biology·Anna C ZemkeBarry R Stripp
Mar 13, 2009·Organogenesis·Stijn P De Langhe, Susan D Reynolds
Feb 2, 2010·American Journal of Respiratory Cell and Molecular Biology·Raksha JainSteven L Brody
Dec 7, 2010·American Journal of Respiratory Cell and Molecular Biology·Moumita GhoshSusan D Reynolds
Jun 1, 2011·Cell Stem Cell·Jason R RockBrigid L M Hogan
Jun 28, 2011·The American Journal of Pathology·Heather M BrechbuhlSusan D Reynolds
Aug 20, 2011·American Journal of Respiratory Cell and Molecular Biology·Russell W SmithSusan D Reynolds
Sep 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Rachel L ZemansGregory P Downey
Oct 12, 2011·The Journal of Clinical Investigation·Thomas VolckaertStijn P De Langhe
Jan 10, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Mary Kathryn SmithSusan D Reynolds
Jan 8, 2015·Development·Munemasa MoriWellington V Cardoso
Sep 1, 2015·The International Journal of Biochemistry & Cell Biology·Fatemeh MoheimaniDarryl A Knight
May 5, 2016·American Journal of Respiratory Cell and Molecular Biology·Susan D ReynoldsMax A Seibold
Apr 19, 2017·Thorax·H A Baarsma, M Königshoff
Sep 15, 2017·British Journal of Pharmacology·Laura MariottiSebastian Guettler

❮ Previous
Next ❯

Citations

Feb 26, 2020·Biochemical Society Transactions·Eszter K Vladar, Melanie Königshoff
Jun 24, 2020·Stem Cells Translational Medicine·Stuart P Atkinson
Aug 28, 2019·Laryngoscope Investigative Otolaryngology·Cynthia M SchwartzSusan D Reynolds
May 22, 2021·Communications Biology·Cody J ArosBrigitte N Gomperts
Jul 13, 2021·Frontiers in Physiology·François M CarlierCharles Pilette
Jul 15, 2021·Pathology Oncology Research : POR·Dingqing FengBin Ling

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.