PMID: 2506433Aug 15, 1989Paper

Regulation of human and murine complement: comparison of 5' structural and functional elements regulating human and murine complement factor B gene expression

Molecular and Cellular Biochemistry
M NonakaH R Colten

Abstract

The serine protease complement factor B (Bf), an acute phase plasma protein, is a component of the alternative pathway of complement activation. Previous studies revealed that several cytokines including IFN-gamma and IL-1 are involved in mediating acute phase Bf expression. To determine the molecular details of Bf expression we isolated, sequenced and characterized the 5' flanking regions of the human and murine Bf genes. In both species the Bf transcriptional start site in liver was located less than 400 bp 3' to the polyadenylation site of the upstream C2 gene. This upstream intergenic region contained greater than 65% nucleotide homology between species. Within this region, an IRS and three heat shock consensus elements were found in the murine sequence in an identical position to that of the human. To examine the functional details of Bf expression, a series of mouse and human Bf promoter-chloramphenicol acetyltransferase (CAT) chimeric gene constructs were transfected into mouse L or human HepG2 cells. Analysis of expression of these fusion gene constructs revealed that 1) cis-acting DNA sequences identified, at least in part, in the 3' untranslated region of the C2 gene (within the 400 bp upstream of the Bf cap site) med...Continue Reading

Citations

Mar 26, 1992·The New England Journal of Medicine·C A JohnsonH R Colten
Dec 18, 2001·European Journal of Immunology·Y HuangB W Winston
May 26, 1998·Scandinavian Journal of Immunology·M PeknaM Pekny
May 29, 1992·Biochemical and Biophysical Research Communications·Y Nagae, U Muller-Eberhard

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