Regulation of human glucocorticoid receptor gene transcription by Sp1 and p53

Molecular and Cellular Endocrinology
Tadashi SuehiroK Hashimoto

Abstract

The human glucocorticoid receptor (hGR) gene has several GC boxes in the promoter 1C region. We studied the effects of Sp1 and p53 on promoter 1C in HepG2 and HEK293 cells using luciferase (Lu) reporter assay. The results showed that the first GC box upstream of the transcription site activated the hGR promoter and over-expression of Sp1 obviously enhanced the activity. A mutant Lu-hGR vector, whose first GC box was defective, lost promoter activity nearly completely. Further, over-expression of p53 strongly suppressed the stimulating effect of Sp1 on hGR promoter activity. We concluded that Sp1 activates the hGR gene promoter, at least in part, by acting on the first GC box in promoter 1C, while p53 suppresses the transactivation by Sp1. These phenomena, demonstrated in cultured cells, may be important for the expression of hGR in vivo.

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Citations

Sep 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Samantha H MurphyInder M Verma
Mar 26, 2011·Journal of Biomedicine & Biotechnology·Levin Böhlig, Karen Rother
Jun 10, 2016·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Katharina GappIsabelle M Mansuy
Aug 26, 2006·The Journal of Biological Chemistry·Diederik Van BodegomSamir S El-Dahr
Feb 6, 2019·Nuclear Receptor Signaling·Rafah MackehTomoshige Kino
May 15, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Xingrao KeSusan S Cohen

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