Regulation of human sperm motility and hyperactivation components by calcium, calmodulin, and protein phosphatases

Archives of Andrology
K AhmadJ S Tash

Abstract

The role of Ca2+, calmodulin, and protein phosphatases on motility and hyperactivation of noncapacitated, capacitating, and detergent-permeabilized reactivated human sperm was examined. In noncapacitated sperm, W7 inhibited percent motility (%MOT), curvilinear velocity (VCL), amplitude of lateral head displacement (ALH), and percent hyperactivation (%HYP) in an extracellular Ca2+ concentration-dependent manner (p < .05). However, in capacitating sperm, inhibition of motility by W7 was independent of external Ca2+. Treatment of reactivated sperm with a synthetic calmodulin inhibitor peptide decreased VCL and ALH in a Ca(2+)-dependent manner (p < .05). Ca2+ exhibited a dramatic influence on motility within a narrow concentration range (0.7 to 1.0 microM) in reactivated sperm. A calmodulin-dependent protein phosphatase (PP2B) was identified by activity assay, immunoblotting, and dephosphorylation of endogenous phosphoproteins. The sperm enzyme, unlike bovine brain PP2B, was inhibited by 1 microM okadaic acid (OA) in the presence of Mn2+, suggesting that the sperm enzyme is unique. In reactivated sperm, inhibition of endogenous PP2B-like activity with anti-PP2B antibodies altered ALH, whereas OA altered both VCL and ALH and also in...Continue Reading

References

Jan 20, 1975·Biochemical and Biophysical Research Communications·F Y Tang, D D Hoskins
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T MuramatsuR L Kincaid
Jan 1, 1991·Cell Motility and the Cytoskeleton·C B LindemannK S Kanous
Sep 30, 1991·Biochemical and Biophysical Research Communications·H MukaiC Tanaka
Jun 1, 1991·Molecular Reproduction and Development·V Giroux-WidemannD Feneux
Mar 31, 1989·Biochemical and Biophysical Research Communications·H IshiharaD Uemura
Jan 1, 1989·Cell Motility and the Cytoskeleton·J S Tash
Nov 1, 1989·Journal of Reproduction and Fertility·C E Stock, L R Fraser
Jun 1, 1986·Biology of Reproduction·W Byrd, D P Wolf
Nov 1, 1987·The Journal of Experimental Zoology·S S SuarezM W Ceglia
Jan 1, 1988·Cell Motility and the Cytoskeleton·C B Lindemann, J S Goltz
May 1, 1988·The Journal of Experimental Zoology·D MortimerA O Trounson
Jan 1, 1987·Methods in Enzymology·J S Tash, A R Means
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D K BlumenthalE G Krebs
Jun 1, 1985·The Journal of Cell Biology·C J Brokaw, S M Nagayama
Jun 1, 1982·Biology of Reproduction·G Swarup, D L Garbers
Jan 1, 1980·The Journal of Cell Biology·B H Gibbons
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H P JonesM J Cormier
Dec 1, 1982·Journal of Pharmacobio-dynamics·S AonumaH Yamada
Jul 1, 1994·Molecular Reproduction and Development·A MújicaS Uribe

❮ Previous
Next ❯

Citations

Jun 22, 2012·PloS One·Ana Hurtado de LleraMaría J Bragado
Jun 13, 2012·Animal Reproduction Science·Jodi LaskoRegina Turner
Jun 19, 2007·Theriogenology·Raffaele BoniElisabetta Tosti
Nov 26, 2003·International Journal of Andrology·K M Williams, W C L Ford
Oct 26, 2016·Molecular Reproduction and Development·Maria Gracia Gervasi, Pablo E Visconti
Oct 26, 2016·Molecular Reproduction and Development·Akihiro KawashimaNaomichi Okamura
May 10, 2005·Biology of Reproduction·George G Ignotz, Susan S Suarez
Feb 20, 1999·Biology of Reproduction·L R JohnsonG L Gerton
Aug 2, 2003·Biology of Reproduction·Heath EcroydR John Aitken
Mar 24, 2016·Scientific Reports·Zhuoran ZhangYu Sun
Apr 9, 2017·Molecular Reproduction and Development·Md Saidur RahmanMyung-Geol Pang
May 7, 1999·Molecular Reproduction and Development·K U DeviS Shivaji
Jun 29, 2018·Journal of Cellular Physiology·Guillermina M LuqueMariano G Buffone
Sep 25, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Fei QuZhide Ding
Jun 12, 2018·The Journal of Reproduction and Development·Lauro González-FernándezMaría Julia Bragado
Apr 20, 2004·Journal of Cellular and Molecular Medicine·G ArientiC A Palmerini
Apr 2, 2019·PloS One·Hossam H ShawkiHisashi Oishi
Mar 20, 2012·Cell and Tissue Research·Janetti SignorelliPatricio Morales
Jun 3, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J S Tash, G E Bracho
Feb 16, 2006·Reproduction, Fertility, and Development·Regina M Turner
Dec 5, 2019·Veterinary Medicine and Science·A R ChaithraShreeShambhudeo D Kharde
Feb 11, 1999·International Journal of Andrology·J SaikhunK Pavasuthipaisit
Aug 8, 1997·Biochemical and Biophysical Research Communications·G E BrachoJ S Tash
Oct 30, 1998·Biochemical and Biophysical Research Communications·J S Tash, G E Bracho
Jan 24, 1998·Biochemical and Biophysical Research Communications·G E BrachoJ S Tash
May 21, 2011·Advances in Medical Sciences·A O MorakinyoO A Adegoke

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.