PMID: 7547684Apr 1, 1995Paper

Regulation of IFN-gamma mRNA production in murine natural killer cells

International Immunology
J A Wilder, D Yuan

Abstract

We have previously reported that large, presumably in vivo activated, B cells stimulate murine natural killer (NK) cells to secrete increased levels of IFN-gamma. In order to further understand the mechanism of IFN-gamma induction, we compared the regulation of IFN-gamma mRNA production after stimulation of NK cells with either B lymphocytes or phorbol myristate acetate (PMA)+ionomycin. Here we show that stimulation of NK cells by either stimuli results increase in IFN-gamma mRNA, albeit with different kinetics. Although the induction requires new RNA synthesis, we could not detect increased transcription of the IFN-gamma gene after stimulation. Measurement of the rate of mRNA degradation after IFN-gamma mRNA has accumulated demonstrates that this mRNA is more stable than IFN-gamma mRNA from unstimulated NK cells. Together, these results suggest that the increase in IFN-gamma mRNA and protein in NK cells, stimulated by B cells or PMA+ionomycin, results from stabilization of pre-existing IFN-gamma message. Our results also suggest that induction of the factor which stabilizes the mRNA, although as yet unknown, requires new RNA synthesis.

Citations

Jun 5, 2010·PLoS Pathogens·Rabinarayan MishraEva Szomolanyi-Tsuda
Mar 29, 2002·Diabetes/metabolism Research and Reviews·N C SchlootH Rothe
Dec 5, 2019·Viral Immunology·Ivayla E GyurovaStephen N Waggoner

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