PMID: 8580664Oct 1, 1995Paper

Regulation of IGF binding proteins by FSH in human luteinizing granulosa cells

Journal of Assisted Reproduction and Genetics
T AdachiY Takeda

Abstract

To evaluate the regulation of the secretion of insulin-like growth factor (IGF) binding proteins (IGFBPs) by FSH in human luteinizing granulosa cells. Luteinizing granulosa cells were incubated with and without FSH. Levels of IGFBP-1 and -3 in the medium were measured by EIA and RIA, respectively and binding activities of these IGFBP were evaluated by Western ligand blot. FSH inhibited the secretion of IGFBP-1 dose dependently. FSH did not inhibit the secretion of immunoreactive IGFBP-3, but inhibited the binding activity of IGFBP-3. To assess the protease activity, 125I-IGFBP-3 was incubated with the cultured medium. 125I-IGFBP-3 degraded into small fragments when it was incubated with the cultured medium treated with FSH. FSH enhances the action of IGF-I in human granulosa cells by inhibiting the secretion of IGFBP-1 and the binding activity of IGFBP-3 by stimulating the proteolysis of IGFBP-3.

References

Feb 11, 1992·The Journal of Clinical Endocrinology and Metabolism·E R HernandezC T Roberts
Apr 1, 1992·The Journal of Clinical Endocrinology and Metabolism·N A Cataldo, L C Giudice
May 1, 1990·Clinical Endocrinology·R KoistinenM Seppälä
Jun 1, 1991·The Journal of Clinical Endocrinology and Metabolism·G LamsonR G Rosenfeld
Oct 1, 1989·The Journal of Clinical Endocrinology and Metabolism·G F EricksonD A Magoffin

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Citations

Nov 30, 2013·Endocrinology and Metabolism Clinics of North America·Anindita Nandi, Leonid Poretsky
Aug 24, 1999·Endocrine Reviews·L PoretskyL C Giudice

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