Regulation of IL-4 gene expression by distal regulatory elements and GATA-3 at the chromatin level

Immunity
Gap Ryol LeeRichard A Flavell

Abstract

Using a transgenic approach, we examined distal regulatory elements located in the IL-4 locus and the role of GATA-3 at these elements. The intergenic DNase I hypersensitive sites (HSS) showed strong enhancement, and the intronic enhancer (IE) and HS5/HS5a sites showed weaker enhancement of the IL-4 promoter. Elements in the 3' region of the IL-4 gene contributed to Th2 specificity. All individual enhancers were T cell activation dependent but not Th2 specific, with the exception of IE. However, when these distal elements were combined into a "minilocus," expression was strongly enhanced and Th2 specific. GATA-3 mediated strong enhancement of IL-4 promoter activity in Th1 cells when the promoter was embedded in the minilocus or linked to HSS and IE, demonstrating that GATA-3 acts through these elements to regulate IL-4 gene expression.

References

Jan 1, 1996·Critical Reviews in Eukaryotic Gene Expression·C BoniferA E Sippel
Oct 31, 1996·Nature·A K AbbasA Sher
Jan 1, 1997·Critical Reviews in Immunology·M A Brown, J Hural
Jan 27, 1998·Immunobiology·M Li-WeberP H Krammer
Mar 11, 1998·Current Opinion in Immunology·S J SzaboI C Ho
May 29, 1998·Current Opinion in Immunology·K M Murphy
Sep 5, 1998·Immunity·J J BirdS L Reiner
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·R BaggaB M Emerson
Sep 24, 1999·Immunologic Research·R A Flavell

❮ Previous
Next ❯

Citations

Jun 29, 2011·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Valéry ElieEvelyne Jacqz-Aigrain
Jun 13, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Samantha SantangeloDontcho Z Staynov
Aug 21, 2013·In Vitro Cellular & Developmental Biology. Animal·Mirim JinMyoung Yun Pyo
Sep 10, 2011·Protein & Cell·Zhenhu LiBing Sun
Apr 26, 2002·Current Opinion in Immunology·Jane L Grogan, Richard M Locksley
Jul 23, 2003·Immunity·Gap Ryol LeeRichard A Flavell
Dec 21, 2010·Nature Immunology·Melanie Van Stry, Mark Bix
Aug 30, 2001·Nature Immunology·H Asnagli, K M Murphy
Aug 30, 2001·Nature Immunology·M MohrsR M Locksley
Nov 2, 2004·Nature Immunology·K Mark AnselAnjana Rao
May 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Byung Hee KohGap Ryol Lee
Dec 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Soo Seok HwangGap Ryol Lee
Apr 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Adam WilliamsRichard A Flavell
May 8, 2002·The Journal of Experimental Medicine·M F NeurathR S Blumberg
Feb 28, 2002·The Journal of Infectious Diseases·Christopher B WilsonMercedes Pérez-Melgosa
May 20, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Yonghua ZhuangHua Huang
Sep 2, 2004·Cold Spring Harbor Symposia on Quantitative Biology·L A PennacchioE M Rubin
Mar 17, 2010·Infection and Immunity·Norihiro HaraguchiNobuyuki Hizawa
Oct 15, 2008·Molecular and Cellular Biology·Andrea L Wurster, Michael J Pazin
Feb 28, 2002·Annual Review of Immunology·Stephen T Smale, Amanda G Fisher
Mar 23, 2004·Annual Review of Immunology·Federica SallustoAntonio Lanzavecchia
Mar 18, 2005·Annual Review of Immunology·Ellen V Rothenberg, Tom Taghon
Mar 23, 2006·Annual Review of Immunology·K Mark AnselAnjana Rao
Mar 23, 2006·Annual Review of Immunology·Catherine V LaiosaThomas Graf
Feb 21, 2002·The Journal of Clinical Investigation·J David FarrarKenneth M Murphy
Apr 17, 2003·The Journal of Clinical Investigation·Len A Pennacchio, Edward M Rubin
Apr 22, 2006·The Journal of Clinical Investigation·Gregory D GregoryMelissa A Brown
Mar 20, 2010·Respiratory Research·Norihiro KikuchiNobuyuki Hizawa
Dec 24, 2013·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Sheikh RayeesGurdarshan Singh
Sep 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Wanda NiedbalaFoo Y Liew
Oct 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Dong U Lee, Anjana Rao
Feb 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Binfeng LuRichard A Flavell
Feb 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jiang F ZhongMichael P Cooke
Oct 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sean T KimRichard A Flavell

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.