Regulation of immunoglobulin gene expression in trans by phorbol esters

European Journal of Immunology
I L MårtenssonT Leanderson

Abstract

Lipopolysaccharide-stimulated B cell cultures treated with phorbol-12,13-dibutyrate have been shown to down-regulate their steady state levels of microseconds RNA while the microns RNA remained constant. In contrast, the steady state levels of delta RNA was up-regulated by the same treatment. By using mice transgenic for a rearranged mu or delta gene, it could be shown that the down-regulatory effect acts in trans on a defined DNA stretch and is independent of isotype. Thus, the positive effect on endogenous delta RNA steady-state levels must be regulated at the level of RNA processing. In addition, a DNA construct containing 1.5 kb of a kappa promoter 5' of, and an IgH enhancer 3' of, a chloramphenicol acetyl transferase reporter gene could be down-regulated in trans by anti-mu- or phorbol-12,13-dibutyrate treatment, showing that transcriptional control of Ig promoter elements can readily be observed in non-transformed B lymphocytes.

References

Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E HögbomT Leanderson
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G GalliJ R Nevins
Oct 1, 1988·Scandinavian Journal of Immunology·E HögbomT Leanderson
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Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·U Chen-BetteckenA Schimpl
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Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard

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Citations

Mar 31, 1998·Scandinavian Journal of Immunology·K AxcronaT Leanderson

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