Regulation of inducible nitric oxide synthase mRNA levels by differentiation and cytokines in human keratinocytes

Biochemical and Biophysical Research Communications
I AranyS K Tyring

Abstract

Inducible nitric oxide synthase (iNOS) transcription is regulated by cytokines and immune stimuli in various cell types. However, there are few data available on iNOS expression and regulation in keratinocytes, even though it can play a role in keratinocyte proliferation during inflammation following wound healing. Our studies demonstrated a differentiation-dependent regulation of iNOS transcription in human keratinocytes from epidermis but not in buccal mucosal cells, which are less differentiated. Different interferon (IFN) types (alpha and gamma) regulated skin keratinocyte iNOS mRNA levels in different ways, predominantly in a differentiation-dependent manner. TGFbeta1 and IFNalpha can antagonize IFNgamma related iNOS induction. However, the antagonistic effects of IFNalpha on IFNgamma differ from those of TGFbeta1. Our studies demonstrate: (a) the presence of iNOS mRNA in human keratinocytes; (b) inducibility of iNOS mRNA by different cytokines; and (c) antagonism between certain cytokines in induction of iNOS mRNA. Further studies are needed to determine biological and therapeutical relevance of our findings.

Citations

Dec 21, 2002·International Journal of Dermatology·Isabelle TenaudBrigitte Dreno
Jul 10, 2002·Experimental Dermatology·László VirágCsaba Szabó
Apr 20, 2012·Free Radical Research·Vania Aparecida TerraAlessandra Lourenco Cecchini
Aug 20, 2003·The Journal of Investigative Dermatology·Johanna M KuchelGary M Halliday
Jan 10, 1998·The Journal of Investigative Dermatology·D Bruch-GerharzV Kolb-Bachofen
Dec 18, 2008·Actas dermo-sifiliográficas·M FerranL F Santamaría-Babi
Nov 3, 2009·Nitric Oxide : Biology and Chemistry·Christoph V SuschekErnst E van Faassen
Sep 4, 2009·The Journal of Investigative Dermatology·Kazunari SugitaYoshiki Tokura
Jul 3, 2007·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Meital PortugalRon Kohen
Apr 14, 2004·Liver International : Official Journal of the International Association for the Study of the Liver·A LegrandG Feldmann
Aug 31, 2006·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·John N CurranDavid Bouchier-Hayes
Mar 7, 2006·Nitric Oxide : Biology and Chemistry·Yi-Chiang HsuWoan-Ruoh Lee
Jun 26, 2003·The Surgical Clinics of North America·Ann Schwentker, Timothy R Billiar
Dec 3, 2014·Journal of Periodontal Research·Q A HussainR P Allaker
Dec 2, 2004·Acta Microbiologica Et Immunologica Hungarica·A PivarcsiA Dobozy
Aug 18, 2001·Toxicology and Applied Pharmacology·M B KabburJ N McDougal
Feb 26, 2003·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Ronald WolfUlrike Blume-Peytavi
May 19, 2004·Archives of Dermatological Research·Andor PivarcsiLajos Kemény
Jan 16, 2019·Advanced Healthcare Materials·Maggie J Malone-PovolnyMark H Schoenfisch
Aug 9, 2007·Dermatology : International Journal for Clinical and Investigative Dermatology·Michael D GoberLaure Aurelian
Oct 27, 2015·Journal of Inflammation·Nir ErdinestAbraham Solomon
Jun 28, 2000·Current Opinion in Clinical Nutrition and Metabolic Care·D T EfronA Barbul
Sep 24, 1998·Immunology Today·R C McKenzie, R Weller
Aug 15, 2002·Nitric Oxide : Biology and Chemistry·Ann SchwentkerTimothy R Billiar
Sep 18, 2002·Journal of Dermatological Science·Young-Jin LimDong-Sup Lee
Sep 19, 2002·Biochemical and Biophysical Research Communications·Manabu YanagitaShinya Murakami
Jul 3, 2021·Vision·Nir ErdinestNadav Levinger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.