Regulation of inhibin subunit gene expression by FSH and estradiol in cultured rat granulosa cells

Endocrinology
I M TurnerS G Hillier

Abstract

Roles of follicle-stimulating hormone (FSH) and sex steroids in regulating the expression of mRNA species encoding the alpha-, beta A- and beta B-subunits of inhibin were studied in cultured granulosa cells from immature rat ovaries. Inhibin subunit mRNAs were detected by Northern blot analysis of total RNA extracted from granulosa cell monolayers which had been incubated for 48 h in serum-free medium containing FSH (100 ng/ml) and/or a steroid (10(-6) M): estradiol (E), testosterone (T) or 5 alpha-dihydrotestosterone (DHT). Levels of mRNA encoding each inhibin subunit in untreated (control) cultures were low. In cultures treated with FSH alone, levels of inhibin alpha-, beta A- and beta B-subunit mRNA were approximately 60-fold, 70-fold and 66-fold greater than control, respectively. In cultures treated with E alone, levels of inhibin alpha- and beta B-subunit mRNA were elevated approximately 4-fold and 2-fold, respectively, but the level of inhibin beta A-subunit mRNA was not measurably affected. Treatment with T or DHT alone had no consistent effect on the levels of any inhibin subunit mRNA. The stimulatory effects of FSH were not consistently altered by the presence of either androgen or estrogen. These results confirm the ...Continue Reading

Citations

Feb 1, 1991·Molecular and Cellular Endocrinology·S G HillierA J Mason
Dec 1, 1992·Molecular and Cellular Endocrinology·J M Ness, B G Kasson
Jul 1, 1993·Molecular and Cellular Endocrinology·C L ChenZ M Feng
Apr 1, 1994·Molecular and Cellular Endocrinology·S G HillierC D Smyth
Jun 23, 2001·Molecular and Cellular Endocrinology·S G Hillier
Apr 12, 2003·Cellular Signalling·Masayuki FunabaMatanobu Abe
Sep 16, 2005·Biology of Reproduction·E E At-TarasJ F Roser
Jul 11, 1991·Clinical Endocrinology·S G HillierA S McNeilly
Apr 4, 2013·Reproductive Biology and Endocrinology : RB&E·Isabel Hernández-OchoaJodi A Flaws
Jul 3, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Rachel C HirstHarry M Charlton
Oct 6, 2000·General and Comparative Endocrinology·A J DavisP A Johnson
May 28, 1993·Annals of the New York Academy of Sciences·S G Hillier, F Miró
Jan 1, 1994·Duodecim; lääketieteellinen aikakauskirja·J Toppari, A Kaipia
Aug 9, 2006·Molecular and Cellular Endocrinology·Nicholas M RobertRobert S Viger
May 12, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Vahid ZadmajidP Mark Lokman
May 20, 2020·Biology of Reproduction·Patricia G Tremblay, Marc-André Sirard
Jul 23, 2014·Endocrine Reviews·Yogeshwar MakanjiTeresa K Woodruff
Jun 1, 1997·Endocrinology·Y ShintaniJ K Findlay
Feb 1, 1996·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·F Scheele, J Schoemaker

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