PMID: 6108561Oct 1, 1980Paper

Regulation of insulin receptors: evidence for involvement of an endocytotic internalization pathway

Proceedings of the National Academy of Sciences of the United States of America
D BaldwinJ M Olefsky

Abstract

Cultured human fibroblasts degrade insulin by a receptor-mediated process. When intracellular hormone degradation is inhibited by chloroquine, 125I-labeled insulin internalizes and accumulates intracellularly. In contrast, cultured IM-9 lymphocytes do not degrade receptor-bound insulin or accumulate 125I-labeled insulin in the presence of chloroquine. Insulin-induced receptor loss occurs in both cell types, and chloroquine inhibits this process in fibroblasts but not in IM-9 lymphocytes. Transglutaminase is a membrane-associated enzyme thought to mediate the process of ligand-induced receptor aggregation and internalization; levels of this enzyme were high in fibroblasts but barely detectable in IM-9 lymphocytes. Furthermore, dansylcadaverine--a potent inhibitor of transglutaminase--blocked insulin-induced receptor loss in fibroblasts but was without effect in IM-9 lymphocytes. These results support the concept that insulin receptor regulation is mediated via an endocytotic internalization pathway in human fibroblasts and that the mechanisms of this process differ among cell types.

References

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Citations

Jan 1, 1984·Molecular and Cellular Biochemistry·P J Davies, M P Murtaugh
Dec 15, 1981·Biochemical and Biophysical Research Communications·Y IwamotoI D Goldfine
May 13, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·S K Ballas, T H Tran
May 1, 1985·Molecular and Cellular Endocrinology·B R LesterF L Stassen
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M FehlmannL Orci
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A Green, J M Olefsky
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R M Smith, L Jarett
Mar 1, 1982·The Journal of Cell Biology·E M MeriskoG E Palade
Nov 1, 1981·Archives of Biochemistry and Biophysics·P A Dennis, N N Aronson
Jul 1, 1982·Metabolism: Clinical and Experimental·J M OlefskyA Green
Jan 17, 1983·The American Journal of Medicine·D H LockwoodM L McCaleb
Jan 1, 1982·Annals of the New York Academy of Sciences·J M OlefskyR L Kroc
May 7, 1991·Journal of Theoretical Biology·M J Quon, L A Campfield
Oct 1, 1985·Diabetes Research and Clinical Practice·H MaegawaY Shigeta
Sep 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Francesca BernassolaGerry Melino
Mar 7, 2021·International Journal of Molecular Sciences·Malcolm A LeissringGermán Perdomo

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