Regulation of interferon-tau (IFN-tau) gene promoters by growth factors that target the Ets-2 composite enhancer: a possible model for maternal control of IFN-tau production by the conceptus during early pregnancy.

Endocrinology
Toshihiko Ezashi, R M Roberts

Abstract

Expression of interferon-tau gene (IFNT) by conceptuses of cattle and sheep must be in phase with the physiological state of the mother if the pregnancy is to be successful. IFNT has a close-to-consensus AP1 site (-71 to -64), overlapping a binding site for Ets-2 (-79 to -70), the key transcription factor controlling IFNT expression. When a bovine IFNT gene control region-luciferase (luc) construct was transfected into mouse 3T3 fibroblasts in the presence of Ets-2 and oncogenic Ras, luc expression was activated (50- to 100-fold). Mutations in either the activator protein 1 (AP1) site or the Ets-2 site of this construct abolished this effect. Similarly, a mutation of Thr72 of the Ets-2 or the addition of a specific inhibitor for the MAPK signal transduction pathway also markedly reduced expression. IFNT promoter activity was up-regulated in response to colony-stimulating factor-1 in 3T3 cells expressing the colony-stimulating factor-1 receptor c-fms. This response did not occur when the AP1 site or the Ets-2 binding sites were mutated. Nor was the response observed in 3T3 cells expressing an inactive form of c-fms. The experiments indicate that IFNT can be activated by growth factors operating through the Ras/MAPK pathway. The ...Continue Reading

Citations

Dec 17, 2009·Reproduction, Fertility, and Development·C E FarinP W Farin
Feb 24, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·D TesfayeM Hoelker
Mar 28, 2009·Theriogenology·Angela M WalkerR Michael Roberts
Nov 23, 2007·Seminars in Cell & Developmental Biology·R Michael RobertsAngela M Walker
Jul 31, 2007·Cytokine & Growth Factor Reviews·R Michael Roberts
Feb 21, 2006·Molecular Reproduction and Development·Kazuhiko ImakawaRonald K Christenson
Jan 16, 2009·American Journal of Reproductive Immunology : AJRI·Alan D Ealy, Qi En Yang
May 1, 2016·BMC Research Notes·Manabu OzawaPeter J Hansen
Oct 7, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Toshihiko Ezashi, Kazuhiko Imakawa
Sep 12, 2007·Reproduction in Domestic Animals = Zuchthygiene·H M Kubisch, K M Johnson
May 16, 2017·Animal Science Journal = Nihon Chikusan Gakkaihō·Kazuhiko ImakawaKazuya Kusama

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