Regulation of interleukin-11 expression in ovulatory follicles of the rat ovary

Reproduction, Fertility, and Development
You-Jee JangMoon-Kyoung Cho

Abstract

The aim of the present study was to examine the regulation of interleukin (IL)-11 expression, as well as the role of IL-11, during ovulation in gonadotropin-primed immature rats. Injection of equine chorionic gonadotropin (eCG), followed by human CG (hCG) to induce superovulation stimulated expression of the Il11 gene in theca cells within 6h, as revealed by northern blot and in situ hybridisation analyses. Real-time reverse transcription-polymerase chain reaction analysis showed that the IL-11 receptor, α subunit gene was expressed in granulosa and theca cells and that injection of hCG had no effect on its expression. IL-11 protein expression was stimulated in theca cells by hCG. LH-stimulated increases in Il11 mRNA levels in cultured preovulatory follicles were inhibited by protein kinase A and mitogen-activated protein kinase kinase inhibitors. Toll-like receptor (TLR) 2 and TLR4 were detected in preovulatory follicles, and the TLR4 ligand lipopolysaccharide, but not the TLR2 ligand Pam3Cys, increased Il11 mRNA levels in theca cells, but not in granulosa cells. Treatment of preovulatory follicles with IL-11 stimulated progesterone production and steroidogenic acute regulatory protein (Star) gene expression. Together, these r...Continue Reading

References

Sep 15, 1995·Genes & Development·J P LydonB W O'Malley
Jun 28, 2002·Molecular Human Reproduction·E DimitriadisL A Salamonsen
Oct 7, 2004·Molecular Human Reproduction·U von RangoI Classen-Linke
Apr 28, 2006·Scandinavian Journal of Immunology·J SchellerS Rose-John
Apr 15, 2008·Trends in Endocrinology and Metabolism : TEM·JoAnne S RichardsMasayuki Shimada
Jan 16, 2009·Biology of Reproduction·Ellen MenkhorstEvdokia Dimitriadis
Jul 2, 2010·Endocrinology·Oliver R OakleyChemyong Ko
May 19, 2011·International Immunopharmacology·Ziyan YangXia Zhang
May 31, 2011·Reproductive Biology and Endocrinology : RB&E·Joanne YapEvdokia Dimitriadis
Jul 21, 2012·Reproductive Sciences·Chang-Ching YehS Joseph Huang
Oct 23, 2012·International Reviews of Immunology·Faezeh Moghimpour BijaniNima Rezaei
Nov 20, 2012·Molecular and Cellular Biochemistry·Takashi ShimizuAkio Miyamoto
Sep 11, 2013·Journal of Leukocyte Biology·Jiajing YinLili Yao
Dec 20, 2013·Clinical Reviews in Allergy & Immunology·Yu LiuQianjin Lu
Apr 16, 2015·Molecular Endocrinology·Hatice S KayaMilan K Bagchi
Jul 1, 2015·Endocrinology·Linah Al-AlemThomas E Curry

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.