Regulation of interleukin-2 and interferon-gamma gene expression in renal failure

Kidney International
L GerezS Shasha

Abstract

Regulated expression of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) genes, induced in cultured peripheral blood mononuclear cells from patients with end-stage renal disease on hemodialysis (HD; N = 13) or peritoneal dialysis (PD; N = 13), was compared to that of 32 normal donors. Culture conditions were chosen that measure the transient, phytohemagglutinin-induced expression of IL-2 and IFN-gamma messenger RNA (mRNA), as well the intactness of post-transcriptional and suppressor T cell-dependent mechanisms that control this expression. The latter was achieved by analyzing the superinduction of IL-2 and IFN-gamma mRNA occurring upon culture with cycloheximide or after low-dose gamma-irradiation, respectively. HD subjects showed a complete loss of inducibility of the IL-2 gene, concomitant with decreased inducibility of IFN-gamma mRNA. In PD subjects, by contrast, expression of IL-2 mRNA was as vigorous as in normal donors, while IFN-gamma mRNA was even more strongly inducible. This difference in gene inducibility is caused by a lack of T cell function in HD subjects. The defect in IL-2 gene expression in HD subjects, occurring most likely at transcription, may underly their impaired immune function.

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Citations

Sep 28, 2007·Seminars in Dialysis·Theodoros EleftheriadisIoannis Stefanidis
Feb 4, 2012·Journal of Innate Immunity·Atsushi SatomuraTomohiro Nakayama
Nov 6, 2013·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Katarzyna A LisowskaJacek M Witkowski
Aug 1, 1994·Kidney International·M K DasguptaP F Halloran
Aug 1, 1993·Kidney International·E LewinK Olgaard
Oct 14, 2004·International Journal of Urology : Official Journal of the Japanese Urological Association·Shigeru SatohTetsuro Kato
Oct 28, 2005·Journal of the American Society of Nephrology : JASN·Wei MuRichard J Johnson
Oct 3, 1999·Journal of the American Society of Nephrology : JASN·B MemoliB Guida
Feb 13, 2001·Kidney International. Supplement·M GirndtH Köhler

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