PMID: 8944732Nov 1, 1996Paper

Regulation of intracellular xanthine oxidase by endothelial-derived nitric oxide

The American Journal of Physiology
C G CoteP M Hassoun

Abstract

We have previously shown that nitric oxide (NO) donors, such as nitrosoglutathione, inhibit endothelial cell (EC) xanthine dehydrogenase (XD)/xanthine oxidase (XO) activity. The purpose of this study was to assess whether endothelial-derived NO plays any role in the regulation of intracellular XD/XO. We exposed rat pulmonary microvascular EC to L-arginine (precursor of NO) or inhibitors of nitric oxide synthase (NOS), i.e., NG-nitro-L-arginine methyl esther (L-NAME) and NG-nitro-L-arginine, in conditions of normoxia, hypoxia, and hypoxia followed by reoxygenation. Hypoxia alone caused a 1.9- and a 6.6-fold increase in XO and a 5-fold increase in XO + XD activities after 24 and 48 h of exposure, respectively. The combination of hypoxia and L-NAME (300 microM) treatment amounted at 48 h to a 10- and 7.5-fold increase in XO and XO + XD activities, respectively, compared with normoxic untreated cells. L-NAME also prevented the decline in XD/XO activity that occurred in untreated EC after hypoxia-reoxygenation. On the other hand, treatment with L-arginine caused a dose-dependent decrease in XD/XO activity in hypoxic EC compared with cells provided with L-arginine-free medium. In separate experiments, we assessed the role of L-argini...Continue Reading

Citations

Apr 25, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Raja-Elie E AbdulnourPaul M Hassoun
Mar 18, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Adel BoueizPaul M Hassoun
Apr 30, 2010·American Journal of Physiology. Cell Physiology·Tiegang LiuUsamah S Kayyali
Aug 8, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kazuyo Yamaji-KeganRoger A Johns
Feb 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Donald Massaro, Gloria D Massaro
Dec 16, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nikki L JerniganBenjimen R Walker
Apr 29, 2005·American Journal of Physiology. Cell Physiology·Steven S AnUsamah S Kayyali
Dec 18, 2012·Biochemistry. Biokhimii︠a︡·V I Kapelko
Sep 12, 2012·Oxidative Medicine and Cellular Longevity·O F Araneda, M Tuesta
Apr 10, 2012·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Tao YangYujian Liu
Apr 15, 2016·American Journal of Physiology. Cell Physiology·Stéphanie ParadisBernard Geny
Feb 8, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Alma Siflinger-Birnboim, Arnold Johnson
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Regina M DayBarry L Fanburg
Jun 13, 2015·Journal of Applied Physiology·Tiegang LiuUsamah S Kayyali
Sep 19, 2006·The International Journal of Biochemistry & Cell Biology·Marian ValkoJoshua Telser

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