PMID: 9159190Jan 1, 1996Paper

Regulation of involucrin gene expression by calcium in normal human keratinocytes

Frontiers in Bioscience : a Journal and Virtual Library
D C NgDaniel D Bikle

Abstract

Calcium is essential for normal epidermal differentiation. Data from Northern and nuclear run-on analysis indicate that involucrin gene transcription is induced by 1.2 mM extracellular calcium. A 3.7 Kbp fragment of the involucrin gene, which contains 2.5 Kbp of upstream region, the transcription start site, and the first intron, was sub-cloned into the pGL3-basic luciferase reporter vector and transfected into pre-confluent normal human keratinocytes (NHK). The stimulated activities of this clone were above basal levels and was further enhanced eight-fold by 1.2 mM extracelluar calcium. The results from a series of truncation and internal deletion experiments revealed multiple calcium-independent enhancer elements between -2476 and -2131 bp of the transcription start site and a calcium-dependent element between -2131 and -2028 bp. This 103 bp fragment contains sequences of an AP-1 site (TGAGTCA), a SP-1 site (GGGCGG), and shares homology with two elements in the human keratin-1 promoter, within the regions identified as mediating the calcium responsiveness of that gene in keratinocytes. One or more of these putative elements may be involved in the calcium-dependent regulation of the involucrin gene transcription in NHK.

Citations

Jul 10, 1999·The Journal of Biological Chemistry·Z Xie, D D Bikle
Feb 7, 2001·The Journal of Biological Chemistry·K HanleyK R Feingold
Aug 16, 2005·The Journal of Investigative Dermatology·M Ernest DoddWendy B Bollag
Oct 18, 2005·Journal of Cellular Biochemistry·Ramakumar Tummala, Satrajit Sinha
May 19, 2000·The Journal of Biological Chemistry·D C NgD D Bikle
Aug 14, 2002·The British Journal of Dermatology·J E Osborne, P E Hutchinson
Mar 16, 2007·Experimental Dermatology·Mónika GöncziLászló Csernoch
Nov 13, 2012·Expert Review of Endocrinology & Metabolism·Daniel D BikleChia-Ling Tu
Jan 1, 2017·The Open Dermatology Journal·Lakiea J BaileyWendy B Bollag
Mar 21, 2019·Cell Death & Disease·Sarmistha MahantySubba Rao Gangi Setty
May 25, 2004·Journal of Cellular Biochemistry·Daniel D Bikle
Dec 1, 2004·The Journal of Nutrition·Daniel D Bikle
Aug 22, 2000·The Journal of Investigative Dermatology·L G KömüvesK R Feingold
Sep 1, 1999·The Journal of Investigative Dermatology·C L TuD D Bikle
Feb 26, 2000·The Journal of Investigative Dermatology·K HanleyK R Feingold
May 4, 2004·Biochemical and Biophysical Research Communications·Elisa Azuara-LiceagaEsther López-Bayghen
Jul 26, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Daniel D BikleZhongian Xie

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