Regulation of ion transport by 5-hydroxytryptamine in rat colon

Clinical and Experimental Pharmacology & Physiology
Yang NingHsiao Chang Chan

Abstract

1. 5-Hydroxytryptamine (5-HT) modulates the motility and secretion of the gastrointestinal tract. To examine the direct effect of 5-HT on the secretions of colonic epithelial cells, a short-circuit current was used to measure electrolyte transport in the rat stripped distal colon. A neuronal Na+ channel blocker and a cyclo-oxygenase inhibitor were routinely added in experiments to abolish the effects of the enteric nervous system and endogenous prostaglandin, respectively. 2. Basolateral application of 5-HT (10 micromol/L) induced an increase in the short circuit current (ISC). Removal of extracellular Cl-, HCO3- or both resulted in a 59.6, 76.4 and 90% reduction of 5-HT-elicited responses, respectively. The Ca(2+)-dependent Cl- channel blocker 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS) had no effect on the 5-HT-induced increase in ISC, but the selective cystic fibrosis transmembrane conductance regulator (CFTR) channel blocker glibenclamide (1 mmol/L) inhibited 5-HT-induced increases in ISC by approximately 92.9%. Removal of apical Na+ reduced the 5-HT-induced increase in ISC by 33.3%. 3. Basolateral pretreatment with 100 micromol/L bumetanide (an inhibitor of the Na(+)-K(+)-2Cl- cotransporter), 200 micromol/L ...Continue Reading

References

Jul 1, 1985·The Journal of Physiology·H AndresJ Schreiner
Feb 14, 1995·European Journal of Pharmacology·D Strabel, M Diener
Jan 13, 1997·Biochemical and Biophysical Research Communications·Y Imada-ShirakataM Okuma
Nov 20, 1997·Comparative Biochemistry and Physiology. Part A, Physiology·M B Hansen, E Skadhauge
Dec 6, 2000·European Journal of Pharmacology·H ItoT Yamaguchi
Mar 20, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·J P LiW Y Chey
Jan 5, 2002·Physiological Reviews·Karl Kunzelmann, Marcus Mall
Mar 13, 2002·Pharmacology, Biochemistry, and Behavior·Daniel HoyerGraeme R Martin
Sep 2, 2003·Gastroenterology·Bi-Guang Tuo, Jon I Isenberg

❮ Previous
Next ❯

Citations

Apr 10, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·D T Beattie, J A M Smith
Sep 2, 2006·Biological & Pharmaceutical Bulletin·Ning YangJin-Xia Zhu
Aug 2, 2007·Biological & Pharmaceutical Bulletin·Hong XueJin Xia Zhu
Jul 11, 2013·World Journal of Gastroenterology : WJG·Evelien K V PriemRomain A Lefebvre
Mar 4, 2008·European Journal of Pharmacology·Ning YangJin-Xia Zhu
Jun 29, 2007·European Journal of Pharmacology·Gui Hong ZhangHsiao Chang Chan
Jun 28, 2007·European Journal of Pharmacology·Erika KällVicente Martinez
Oct 15, 2009·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·A V EmmanuelN J Talley
Jun 11, 2015·British Journal of Pharmacology·I KajiJ D Kaunitz
Oct 16, 2014·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M GiletL Bueno
Jul 13, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Xiaofang LuJing Xie
Apr 15, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yogesh BhattaraiPurna C Kashyap
Dec 6, 2017·World Journal of Gastroenterology : WJG·Xiao-Fang LuLu-Qing Zhao
Aug 9, 2006·European Journal of Gastroenterology & Hepatology·Robert MaderMoriko Ito
Sep 17, 2019·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Rachel M Gwynne, Joel C Bornstein
Dec 5, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anders J SmithHui Dong
Feb 13, 2021·Medical Sciences : Open Access Journal·Randal K BuddingtonScott C Howard

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.