Regulation of ion transport by pH and [HCO3-] in isolated gills of the crab Neohelice (Chasmagnathus) granulata

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Martin TresguerresCarlos M Luquet

Abstract

Posterior isolated gills of Neohelice (Chasmagnathus) granulatus were symmetrically perfused with hemolymph-like saline of varying [HCO3-] and pH. Elevating [HCO3-] in the saline from 2.5 to 12.5 mmol/l (pH 7.75 in both cases) induced a significant increase in the transepithelial potential difference (Vte), a measure of ion transport. The elevation in [HCO3-] also induced a switch from acid secretion (-43.7 +/- 22.5 microequiv.kg(-1).h(-1)) in controls to base secretion (84.7 +/- 14.4 microequiv.kg(-1).h(-1)). The HCO3(-)-induced Vte increase was inhibited by basolateral acetazolamide (200 micromol/l), amiloride (1 mmol/l), and ouabain (5 mmol/l) but not by bafilomycin (100 nmol/l). The Vte response to HCO3(-) did not take place in Cl(-)-free conditions; however, it was unaffected by apical SITS (2 mmol/l) or DIDS (1 mmol/l). A decrease in pH from 7.75 to 7.45 pH units in the perfusate also induced a significant increase in Vte, which was matched by a net increase in acid secretion of 67.8 +/- 18.4 microequiv kg(-1) h(-1). This stimulation was sensitive to basolateral acetazolamide, bafilomycin, DIDS, and Na+-free conditions, but it still took place in Cl(-)-free saline. Therefore, the cellular response to low pH is different f...Continue Reading

References

Oct 1, 1989·The American Journal of Physiology·R E Shetlar, D W Towle
Jun 18, 1996·Biochemistry·K FendlerE Bamberg
Jan 1, 1996·Annual Review of Physiology·R P Henry
Feb 13, 2003·The Journal of Experimental Biology·Horst OnkenCarlos M Luquet
Jun 5, 2003·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Cedomil Lucu, David W Towle
Mar 24, 2004·The Journal of Experimental Biology·Leonard B Kirschner
Aug 3, 2004·Physiological and Biochemical Zoology : PBZ·I-Chi Chang, Pung-Pung Hwang
Oct 9, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·J HalperinC M Luquet
Dec 8, 2004·The Journal of Experimental Biology·Dirk WeihrauchDavid W Towle
Oct 1, 2005·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·G GenoveseC M Luquet
Jun 17, 2006·Respiratory Physiology & Neurobiology·S F Perry, K M Gilmour
Jul 22, 2006·The Journal of Experimental Biology·Martin Grosell
Sep 2, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Martin TresguerresGreg G Goss
Oct 17, 2006·Physiological and Biochemical Zoology : PBZ·Martin TresguerresGreg G Goss
Jan 6, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Martin TresguerresGreg G Goss
Feb 3, 2007·The Journal of Experimental Biology·Jyuan-Ru Tsai, Hui-Chen Lin
May 22, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Martin TresguerresGreg G Goss
Jul 3, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Carolina A FreireJohn C McNamara

❮ Previous
Next ❯

Citations

Apr 27, 2011·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Khuong Tuyen HuynhColin J Brauner
Apr 23, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Martin TresguerresMartin Grosell
May 9, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Daniela P GarçonFrancisco A Leone
Jun 19, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Douglas C MasuiFrancisco A Leone
Jun 28, 2014·Biochimica Et Biophysica Acta·Martin Tresguerres
May 28, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kelly Cristina Silva FirminoRosa Prazeres Melo Furriel
Oct 2, 2012·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Sandra Fehsenfeld, Dirk Weihrauch
Jul 23, 2016·The Journal of Experimental Biology·Martin Tresguerres
Aug 6, 2020·Frontiers in Physiology·Weichuan LinChunlin Wang
Apr 19, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Scott K ParksGreg G Goss

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
Carolina A FreireJ C McNamara
The Journal of Experimental Biology
Dirk WeihrauchDavid W Towle
Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
C Lucu, David W Towle
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
R P M FurrielF A Leone
© 2021 Meta ULC. All rights reserved