PMID: 9660174Jul 11, 1998Paper

Regulation of iron metabolism in the sanguivore lamprey Lampetra fluviatilis--molecular cloning of two ferritin subunits and two iron-regulatory proteins (IRP) reveals evolutionary conservation of the iron-regulatory element (IRE)/IRP regulatory system

European Journal of Biochemistry
O AndersenV Pieribone

Abstract

Two ferritin cDNAs were cloned from the liver and spinal cord of the sanguivore lamprey Lampetra fluviatilis, an extant representative of the ancient agnathan (jawless) stage in vertebrate evolution. The deduced proteins of 20.2 kDa (H-subunit) and 20.1 kDa (M-subunit) display 73% sequence identity, and both contain the ferroxidase center characteristic of animal H-ferritin. A highly conserved iron-responsive element (IRE) was identified in the 5' untranslated region of lamprey H-ferritin. Lamprey ferritin IRE forms a specific complex with crude lamprey and rat liver extracts, and with recombinant human iron-regulatory protein (IRP-1) in an electrophoretic mobility shift assay. Furthermore, lamprey ferritin IRE competes with labeled human ferritin IRE for binding to IRP in lamprey and mammalian extracts. Two liver cDNA sequences encoding 323 residues and 101 residues of two genetically distinct lamprey IRP were amplified by PCR. Lamprey IRP-1 and IRP-2, which are 72% identical, display about 74% sequence identity to their presumed homologues in mammals. Northern blot analysis shows that two IRP transcripts of 3.6 kb and 5.8 kb are expressed in lamprey liver. Given the ancient lineage of lampreys, the results indicate that the I...Continue Reading

Citations

Oct 12, 2012·Marine Biotechnology·Carmen García FernándezElena Sarropoulou
Mar 5, 2003·Molecular and Biochemical Parasitology·Mark LoyevskyTracey A Rouault
Nov 22, 2000·The Biochemical Journal·G Cairo, A Pietrangelo
Jul 8, 2008·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Ying ZhangGuang-wei Zhong
Dec 26, 2015·Biomolecules·Elisa E Figueroa-AnguloRossana Arroyo
Dec 8, 2011·Fish & Shellfish Immunology·Juan LiGuofan Zhang
Apr 13, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Wen-jiang ZhengLi Sun
Mar 26, 2010·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Hong LiuZhanjiang Liu
Nov 27, 2015·Fish Physiology and Biochemistry·Volodymyr I Lushchak
May 20, 2015·Fish Physiology and Biochemistry·Jing-Jing Wang, Li Sun
Feb 25, 2014·Fish & Shellfish Immunology·Martha Reyes-BecerrilFelipe Ascencio-Valle
Aug 6, 2011·Rapid Communications in Mass Spectrometry : RCM·Bo ZhuHe-Qing Huang

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