Regulation of Iron Storage by CsrA Supports Exponential Growth of Escherichia coli

MBio
Christine PourciauTony Romeo

Abstract

The global regulatory protein CsrA coordinates gene expression in response to physiological cues reflecting cellular stress and nutrition. CsrA binding to the 5' segments of mRNA targets affects their translation, RNA stability, and/or transcript elongation. Recent studies identified probable mRNA targets of CsrA that are involved in iron uptake and storage in Escherichia coli, suggesting an unexplored role for CsrA in regulating iron homeostasis. Here, we assessed the impact of CsrA on iron-related gene expression, cellular iron, and growth under various iron levels. We investigated five new targets of CsrA regulation, including the genes for 4 ferritin or ferritin-like iron storage proteins (ISPs) and the stress-inducible Fe-S repair protein, SufA. CsrA bound with high affinity and specificity to ftnB, bfr, and dps mRNAs and inhibited their translation, while it modestly activated ftnA expression. Furthermore, CsrA was found to regulate cellular iron levels and support growth by repressing the expression of genes for ISPs, most importantly, ferritin B (FtnB) and bacterioferritin (Bfr). Iron starvation did not substantially affect cellular levels of CsrA or its small RNA (sRNA) antagonists, CsrB and CsrC. csrA disruption led t...Continue Reading

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Citations

Oct 14, 2020·The Journal of Biological Chemistry·Justin M BradleyNick E Le Brun
Feb 9, 2021·Frontiers in Microbiology·Mikel Irastortza-Olaziregi, Orna Amster-Choder
Feb 4, 2021·MBio·Heidi A ButzShelley M Payne
Nov 17, 2020·Frontiers in Microbiology·Christine PourciauTony Romeo
Jan 18, 2021·The Journal of Biological Chemistry·Justin M BradleyNick E Le Brun
Jul 13, 2021·MicroLife·Olivier Cunrath, Jacob D Palmer
Jun 10, 2021·Molecular Microbiology·Ying-Jung LaiTony Romeo

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Methods Mentioned

BETA
CLIP-seq
footprinting
PCR
gel mobility shift
in
in vitro transcription
electrophoresis
protein assay

Software Mentioned

Quantity One
EMSA

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