Regulation of iron uptake in primary culture rat hepatocytes: the role of acute-phase cytokines

Shock
Shakil AhmadIhtzaz Ahmed Malik

Abstract

Decreased serum and increased hepatic iron uptake is the hallmark of acute-phase (AP) response. Iron uptake is controlled by iron transport proteins such as transferrin receptors (TfRs) and lipocalin 2 (LCN-2). The current study aimed to understand the regulation of iron uptake in primary culture hepatocytes in the presence/absence of AP mediators. Rat hepatocytes were stimulated with different concentrations of iron alone (0.01, 0.1, 0.5 mM) and AP cytokines (interleukin 6 [IL-6], IL-1β, tumor necrosis factor α) in the presence/absence of iron (FeCl3: 0.1 mM). Hepatocytes were harvested at different time points (0, 6, 12, 24 h). Total mRNA and proteins were extracted for reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot. A significant iron uptake was detected with 0.1 mM iron administration with a maximum (133.37 ± 4.82 µg/g of protein) at 24 h compared with control and other iron concentrations. This uptake was further enhanced in the presence of AP cytokines with a maximum iron uptake (481 ± 25.81 µg/g of protein) after concomitant administration of IL-6 + iron to cultured hepatocytes. Concomitantly, gene expression of LCN-2 and ferritin subunits (light- and heavy-chain ferritin subunits) was upregula...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1990·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·G RamadoriK H Meyer zum Büschenfelde
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R S EisensteinH N Munro
Jul 28, 1999·Seminars in Liver Disease·G Ramadori, B Christ
Jun 6, 2003·American Journal of Physiology. Renal Physiology·Jun YangJonathan Barasch
Sep 4, 2003·The Journal of Cell Biology·Sean D Conner, Sandra L Schmid
Apr 28, 2004·Cell·Matthias W HentzeNancy C Andrews
Jul 22, 2004·Analytical Biochemistry·Jan RiemerRalf Dringen
Oct 7, 2004·Biological Trace Element Research·Qiong LiuDeding Tao
Jan 11, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Kyrylo TronGiuliano Ramadori
Apr 1, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Nadeem SheikhGiuliano Ramadori
Jan 19, 2007·Journal of the American Society of Nephrology : JASN·Kai M Schmidt-OttJonathan Barasch
Apr 13, 2007·Blood Cells, Molecules & Diseases·Alessia CalzolariUgo Testa
Dec 28, 2007·The Journal of Biological Chemistry·Mimi C SammarcoEd Grabczyk
Oct 22, 2008·Biochimica Et Biophysica Acta·Paolo ArosioPatrizia Cavadini
Apr 15, 2011·Anemia·Eugenia Prus, Eitan Fibach
Apr 4, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Naila NazGiuliano Ramadori
May 4, 2012·Cold Spring Harbor Perspectives in Medicine·Tomas Ganz, Elizabeta Nemeth
Jul 13, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gayathri SrinivasanMatam Vijay-Kumar
May 3, 2013·PloS One·Jean-Christophe Deschemin, Sophie Vaulont

❮ Previous
Next ❯

Citations

Mar 19, 2014·Shock·Mark G Clemens
May 3, 2016·International Journal of Molecular Sciences·Sadaf SultanSilke Cameron
Jul 19, 2016·Metallomics : Integrated Biometal Science·M C Linder
Dec 13, 2016·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Salih CetinkayaAyse Kirbas
May 9, 2018·Current Neuropharmacology·Bo Wang, Xiao-Ping Wang
Mar 19, 2020·The European Respiratory Journal·Md Khadem AliJay C Horvat
Nov 21, 2017·World Journal of Gastroenterology : WJG·Ihtzaz Ahmed MalikNaila Naz
Dec 21, 2016·Archives of Toxicology·Salamah Mohammad Alwahsh, Rolf Gebhardt
Apr 23, 2020·Oxidative Medicine and Cellular Longevity·Tasleem AkhtarNadeem Sheikh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.