Abstract
Intracellular insulin signaling involves a series of alternative and complementary pathways created by the multiple substrates of the insulin receptor (IRS) and the various isoforms of the SH2 domain signaling molecules that can interact with substrate. In this study we investigated IRS-1 and IRS-2 tyrosine phosphorylation, their association with PI3-kinase and the phosphorylation of Akt, a serine-threonine kinase situated downstream to PI 3-kinase, in liver and muscle of two animal models of insulin resistance: 72 h of fasting and STZ-diabetic rats. There was an upregulation in insulin-induced IRS-1 and IRS-2 tyrosine phosphorylation and association with PI3-kinase in liver and muscle of both animal models of insulin resistance. However, Akt phosphorylation showed different regulation, increasing in fasting and decreasing in STZ-diabetic rats. Since an important difference between these two animal models of insulin resistance is the plasma glucose levels, we can suggest that in STZ diabetic rats, the reduction in Akt phosphorylation is probably related to hyperglycemia and may certainly contribute to the molecular mechanism of insulin resistance observed in these animals.
References
Oct 1, 1975·The Journal of Clinical Investigation·A H SoliJ Roth
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1979·Endocrinology·E C Almira, W J Reddy
Nov 1, 1992·The Journal of Clinical Investigation·M J SaadC R Kahn
Jul 4, 1991·Nature·X J SunM F White
Feb 1, 1991·The Journal of Clinical Investigation·B B KahnL Rossetti
Nov 10, 1994·Nature·E ArakiC R Kahn
Aug 17, 1995·Nature·B M Burgering, P J Coffer
Sep 14, 1995·Nature·X J SunM F White
Oct 1, 1993·The Journal of Clinical Investigation·F FolliC R Kahn
Oct 1, 1993·The Journal of Clinical Investigation·M J SaadC R Kahn
Mar 31, 1995·Science·R Yao, G M Cooper
Mar 1, 1994·Diabetologia·J R ZierathH Wallberg-Henriksson
Jan 26, 1996·The Journal of Biological Chemistry·R A GabbayJ S Flier
Feb 8, 1996·Nature·M Holgado-MadrugaA J Wong
Jun 1, 1996·Molecular and Cellular Biology·T YamauchiT Kadowaki
Jan 1, 1996·Annual Review of Pharmacology and Toxicology·M G Myers, M F White
Nov 29, 1996·The Journal of Biological Chemistry·K DattaP N Tsichlis
Dec 6, 1996·The Journal of Biological Chemistry·A D KohnR A Roth
Jan 1, 1997·Molecular and Cellular Biology·A KlippelL T Williams
Jan 31, 1997·Science·H DudekM E Greenberg
Mar 1, 1997·Molecular and Cellular Biology·G KulikM J Weber
Jun 25, 1998·Molecular and Cellular Biology·T KitamuraM Kasuga
Citations
Jun 24, 2008·Cardiovascular Diabetology·Marlene F Shehata
Apr 10, 2008·Cardiovascular Diabetology·Marlene F Shehata
Jun 30, 2012·PloS One·Samir SofticRohit Kohli
Mar 11, 2004·American Journal of Physiology. Endocrinology and Metabolism·Aidar R GosmanovDonald B Thomason
Jun 18, 2004·The Journal of Biological Chemistry·Tatsuya Kondo, C Ronald Kahn
Jun 30, 2007·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Guillermo E UmpierrezDonald Thomason
Nov 16, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Aidar R GosmanovDonald B Thomason
Jul 16, 2019·Experimental Gerontology·Mei LuTan Wang