PMID: 9645969Jul 1, 1998Paper

Regulation of kappa-opioid receptor mRNA level by cyclic AMP and growth factors in cultured rat glial cells

Brain Research. Molecular Brain Research
P Tryoen-TothG Labourdette

Abstract

The mRNA of the kappa-opioid receptor (KOR) has been found recently in cultured astrocytes and in microglia. By using RT-PCR and Southern hybridization, we confirmed these observations and, in addition, we observed that KOR mRNA was expressed in oligodendrocytes and in the precursors of astrocytes and oligodendrocytes. KOR mRNA level was the highest in the immature astrocytes and decreased with their maturation. Very few data are available on the regulation of KOR level by extracellular signals. Therefore, we examined the effect of three growth factors known to be present in the adult brain, basic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF-BB) and leukemia inhibitory factor (LIF) and of two cyclic AMP (cAMP) generating systems, the cAMP analog, 8-(4-chlorophenylthio)-cAMP and forskolin, on this level. It was found that in astrocytes, KOR mRNA level decreased dramatically under the effect of cAMP and less under the effect of bFGF while it did not change significantly after LIF treatment. In oligodendrocytes, it also decreased with cAMP, but increased under the effect of bFGF and PDGF-BB. In microglia, a decrease was observed with cAMP and lipopolysaccharides (LPS), the most used activators of macrophag...Continue Reading

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Citations

Mar 21, 2000·Journal of Neuroscience Research·P Tryoen-TothG Labourdette
Jul 3, 2003·Molecular and Cellular Neurosciences·Anders I PerssonPeter S Eriksson
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·P Y LawH H Loh
Feb 26, 2008·Neurochemistry International·Tsung-Kuei KaoChun-Jung Chen

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