Regulation of KCNQ1/KCNE1 by β-catenin

Molecular Membrane Biology
Jan WilmesFlorian Lang

Abstract

β-catenin, a multifunctional protein expressed in all tissues including the heart stimulates the expression of several genes important for cell proliferation. Signaling involving ß-catenin participates in directing cardiac development and in the pathophysiology of cardiac hypertrophy. Nothing is known, however, on the role of β-catenin in the regulation of cardiac ion channels. The present study explored the functional interaction of β-catenin and KCNE1/KCNQ1, the K⁺ channel complex underlying the slowly activating outwardly rectifying K⁺ current. To this end, KCNE1/KCNQ1 was expressed in Xenopus oocytes with and without β-catenin and the depolarization (up to + 80 mV) induced current (I(Ks)) was determined using the two-electrode voltage clamp. As a result, β-catenin enhanced I(Ks) by 30%. The effect of β-catenin on I(Ks) was not affected by actinomycin D (10 μM), an inhibitor of transcription, indicating that β-catenin was not effective as transcription factor. Confocal microscopy revealed that β-catenin enhanced the KCNE1/KCNQ1 protein abundance in the cell membrane. Exposure of the oocytes to brefeldin A (5 μM), an inhibitor of vesicle insertion, was followed by a decline of I(Ks), which was then similar in oocytes expressi...Continue Reading

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Citations

Aug 21, 2012·PloS One·Carlos MunozFlorian Lang
Nov 20, 2016·Acta Physiologica·S Bandulik
Nov 28, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Yongchao MouJin Zhou
Oct 12, 2018·Current Medicinal Chemistry·Mentor SopjaniMiribane Dërmaku-Sopjani
Oct 23, 2019·World Journal of Gastroenterology : WJG·Kyle J AndersonPatricia M Scott

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