PMID: 8950452Nov 1, 1996Paper

Regulation of keratinocyte proliferation and differentiation by all-trans-retinoic acid, 9-cis-retinoic acid and 1,25-dihydroxy vitamin D3

Archives of Dermatological Research
S GibbsM Ponec

Abstract

We studied the effect of all-trans retinoic acid (all-trans-RA), 9-cis-retinoic acid (9-cis-RA) and 1,25-dihydroxy vitamin D3 (1,25(OH)2D3) on proliferation and differentiation of human keratinocytes cultured in a submerged culture system for up to 5 weeks and evaluated changes in cell morphology and in the expression of proliferation- and terminal differentiation-related genes on both the mRNA and the protein levels. Under control culture conditions, the expression of small proline-rich proteins (SPRR1 and SPRR2), involucrin, Ki67 and c-jun reached a maximum after 2 weeks in culture (1 week postconfluence) and then decreased as the tissue architecture of the cultures deteriorated. Upon simultaneous treatment with both retinoids and 1,25(OH)2D3 a culture was generated that remained stable for 4 weeks with at least eight living cell layers. Furthermore, this culture showed a pattern of SPRR2 and involucrin expression which closely resembled that of native epidermis, a maintained Ki67 expression and a strongly induced c-jun expression. Treatment with 1,25(OH)2D3 alone inhibited cell proliferation and stimulated cell differentiation resulting in acceleration of the differentiated phenotype and was accompanied by inhibition of c-ju...Continue Reading

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Citations

Jun 5, 1999·Cell Biochemistry and Biophysics·J Tesfaigzi, D M Carlson
Sep 18, 2010·Mediators of Inflammation·Aswin HariP Régine Mydlarski
Sep 18, 2009·The Journal of Investigative Dermatology·Enikö SonkolyAndor Pivarcsi
Jun 3, 2006·The Journal of Investigative Dermatology·Jay G Gao, Marcia Simon
Mar 21, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hyunjoo J LeeKenneth H Albrecht
May 23, 2012·The British Journal of Dermatology·N IrlaN Yawalkar
Feb 26, 2013·Journal of the European Academy of Dermatology and Venereology : JEADV·J A JohnsonA W Armstrong
Aug 16, 2016·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Vivek M GhateNilkumar Patel
Jul 14, 2016·Tissue Engineering. Part C, Methods·Jeroen K BuskermolenSusan Gibbs
Jul 16, 2009·Experimental Dermatology·Andreas KerstanAxel Trautmann
Apr 11, 2019·Journal of Tissue Engineering and Regenerative Medicine·Charlotte Rodrigues NevesSusan Gibbs
Mar 18, 2011·Experimental Dermatology·Johanna MihályRalph Rühl
Feb 25, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philip T LiuRobert L Modlin
Jul 5, 2011·Biochemical and Biophysical Research Communications·Cristina Velez-DelValleWalid Kuri-Harcuch

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