Regulation of KLF12 by microRNA-20b and microRNA-106a in cystogenesis

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Yubin ShinJong Hoon Park

Abstract

Autosomal dominant polycystic kidney disease (ADPKD) is one of the most common inherited disorders. ADPKD is caused by mutations in the gene encoding either polycystic kidney disease 1 ( PKD1) or polycystic kidney disease 2 ( PKD2). Patients with ADPKD show progressive growth of cystic fluid-filled renal cysts. Here, we used Pkd2f/f control mice and Pkd2f/f:HoxB7-Cre experimental mice, which are bred to have a conditional deletion of Pkd2 in the collecting ducts, and analyzed the expression pattern of microRNAs (miRNAs) of kidney tissues from Pkd2f/f and Pkd2f/f:HoxB7-Cre mice. Decreased expression of miR-20b-5p and miR-106a-5p in Pkd2f/f:HoxB7-Cre mice compared to that in Pkd2f/f mice was observed. These miRNAs target Klf12 (Krüppel-like factor 12), which has low expression in kidney tissues of Pkd2f/f mice; however, its expression is enhanced in Pkd2f/f:HoxB7-Cre mice over time. Moreover, miR-20b-5p and miR-106a-5p directly target Klf12 mRNA by binding to the 3'-UTR of Klf12. In addition, human and mouse cell lines exhibit similar patterns. These findings were also consistent with the data from Pkd2 knockout mouse embryonic fibroblasts. Furthermore, direct and indirect knockdown of Klf12 slows cyst growth and cell proliferati...Continue Reading

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Oct 28, 2017·Scientific Reports·Yu Mi WooJong Hoon Park

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Citations

Oct 18, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Do Yeon KimJong Hoon Park
Jul 14, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Viola C LamLewis L Lanier
Dec 26, 2018·Clinical Kidney Journal·Thomas WeimbsBryan C Bourgeois
Apr 4, 2021·International Journal of Molecular Sciences·Julie Xia Zhou, Xiaogang Li

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