Regulation of L-cystine transport in Salmonella typhimurium.

Journal of Bacteriology
E W Baptist, N M Kredich

Abstract

A kinetic analysis of L-cystine uptake in wild-type Salmonella typhimurium indicates the presence of at least two, and possibly three, separate transport systems. CTS-1 accounts for the majority of uptake at 20 muM L-cystine, with a Vmax of 9.5 nmol/min per mg and a Km of 2.0 muM; CTS-2 is a low-capacity, higher-affinity system with a Vmax of 0.22 nmol/min per mg and a Km of 0.05 muM; a third, nonsaturable process has been designated CTS-3. We find that wild-type CTS-1 levels are at least 11 times higher in sulfur-limited cells than in L-cystine-grown cells. Pleiotropic cysteine auxotrophs of the types cysE (lacking serine transacetylase) and cysB- (lacking a regulatory element of positive control) have very low levels of CTS-1 even when grown under conditions of sulfur limitation, which response is analogous to that previously observed for cysteine biosynthetic enzymes (N . M. Kredich, J. Biol. Chem. 246:3474-3484, 1971). CTS-1 is induced in cysE mutants by growth in the presence of O-acetyl-L-serine (the product of serine transacetylase), again paralleling the behavior of the cysteine biosynthetic pathway. Strain DW25, a prototrophic cysBc mutant, which is constitutive for cysteine biosynthesis, is also derepressed for CTS-1 ...Continue Reading

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Citations

Jul 21, 2004·Journal of Bacteriology·Pierre BurguièreIsabelle Martin-Verstraete
Sep 10, 2015·Journal of Bacteriology·Karin R Chonoles ImlayJames A Imlay
Sep 20, 1978·Molecular & General Genetics : MGG·G Jagura, D Hulanicka
Jun 6, 2006·Applied and Environmental Microbiology·Fanny Ewann, Paul S Hoffman
Nov 20, 2013·Bioprocess and Biosystems Engineering·Sung Hun YounChul Soo Shin
Oct 28, 2014·Bioscience, Biotechnology, and Biochemistry·Kaneyoshi YamamotoAkira Ishihama
Apr 22, 2011·The Journal of Biological Chemistry·Amit Kumar Yadav, Anand Kumar Bachhawat
Jun 26, 2001·Research in Microbiology·M A Kertesz
Jun 1, 1978·Microbiological Reviews·K E Sanderson, P E Hartman
Nov 1, 2005·EcoSal Plus·Larry Reitzer
Sep 1, 2008·EcoSal Plus·Nicholas M Kredich

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