Regulation of Laccase Gene Transcription in Trametes versicolor.

Applied and Environmental Microbiology
P J Collins, A Dobson

Abstract

The expression of laccase in the white rot fungus Trametes versicolor is regulated at the level of gene transcription by copper and nitrogen. We used reverse transcription-PCR to demonstrate that as the concentration of copper or nitrogen in fungal cultures was increased, an increase in laccase activity, corresponding to increased laccase gene transcription levels, was observed. In addition, we demonstrated that the amounts of laccase mRNA and laccase activity in 10-day-old cultures were a direct function of the concentration of either 1-hydroxybenzotriazole, a previously described laccase substrate, or 2,5-xylidine, a well-known laccase inducer, in the medium. No induction was observed after the addition of two aromatic acids, ferulic acid and veratric acid, which have been shown to induce laccase production in other white rot fungi. When either copper, 2,5-xylidine, or both compounds were added to cultures grown in the absence of copper, increased laccase transcript levels were detected within 15 min. Corresponding increases in laccase activity were observed after 24-h incubation only when copper was present.

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Citations

Dec 29, 2011·Applied Biochemistry and Biotechnology·Shiv Shankar, Shikha
Jan 3, 2012·Applied and Environmental Microbiology·S CamareroM Alcalde
Jan 14, 2004·European Journal of Biochemistry·Shicheng ChenJohn A Buswell
Mar 2, 1999·Applied and Environmental Microbiology·P J CollinsA D Dobson
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Jan 23, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xinping LiuYang Yang
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