Abstract
Genes that encode 3rd instar larval cuticle proteins (LCP's) of Drosophila melanogaster are located in at least two chromosomal sites. The genes encoding four of the five predominant LCP's are located in a cluster at the chromosomal region 44D. They are organized in pairs that are transcribed divergently, and expressed with different timing during the third larval instar. Towards understanding the basis of gene regulation within the 44D cluster, we have analyzed genetic variants, including the 2-3 variant, which has an insertion of a copia-like transposable element, H.M.S. Beagle, within the 44D cluster. The Beagle element appears to inactivate the LCP-3 gene by inserting into its TATA box, but also may cause the precocious expression of two other LCP genes, LCP-1 and LCP-f2, in the cluster. The long terminal repeat (LTR) of the Beagle element apparently contains a sequence, perhaps an enhancer-like element, which causes altered expression of these genes. We have also investigated the cis-regulatory elements involved in expression of the LCP-2 gene in wild-type larvae. We have identified two upstream regions that may contain separate cis-regulatory elements. The region between -252 bp and -515 bp may be essential for any expres...Continue Reading
References
Sep 19, 1978·Biochemistry·J W FristromF Watt
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 27, 1979·Biochemistry·J M ChirgwinW J Rutter
Jun 3, 1988·Cell·M D Biggin, R Tjian
Aug 1, 1987·Genetics·D Mismer, G M Rubin
Oct 1, 1986·Genetics·C J Chihara, D A Kimbrell
Oct 22, 1987·Nature·C N ChenR L Davis
Jun 3, 1988·Cell·M D BigginR Tjian
Aug 13, 1974·Biochemistry·R D Palmiter
Aug 1, 1984·Cell·R E Karess, G M Rubin
Dec 11, 1984·Nucleic Acids Research·J C EissenbergS C Elgin
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Jul 1, 1981·Cell·M SnyderN Davidson
Feb 1, 1982·Developmental Biology·C J ChiharaJ W Fristrom
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·V CorcesM Meselson
Dec 1, 1981·Cell·J BanerjiW Schaffner
Oct 22, 1982·Science·A C Spradling, G M Rubin
Oct 22, 1982·Science·G M Rubin, A C Spradling
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M P SnyderN Davidson
Feb 11, 1983·Science·H WeiherP Gruss
Sep 24, 1983·Nucleic Acids Research·G M Rubin, A C Spradling
Jan 1, 1984·Annual Review of Genetics·M R Goldsmith, F C Kafatos
Apr 1, 1983·Molecular and Cellular Biology·T E CrowleyE M Meyerowitz
Dec 1, 1983·Cell·J T LisC A Sutton
Nov 20, 1984·Biochemistry·D J SilvertJ W Fristrom
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S C WadsworthB J McCarthy
Oct 1, 1980·Cell·T BarnettP C Wensink
Jun 1, 1982·Developmental Biology·J W FristromD J Silvert
Jul 1, 1982·Cell·M SnyderN Davidson