Regulation of lipid-induced macrophage polarization through modulating peroxisome proliferator-activated receptor-gamma activity affects hepatic lipid metabolism via a Toll-like receptor 4/NF-κB signaling pathway.

Journal of Gastroenterology and Hepatology
Hui-Min WuJ Hua

Abstract

Chronic inflammation links closely to insulin resistance and lipid metabolism in nonalcoholic fatty liver disease (NAFLD). Macrophage M1 activation plays an important role in the initiation and continuing of pro-inflammatory response of NAFLD. Our study was to investigate whether macrophage M1/M2 polarization switching would affect hepatic inflammation and lipid metabolism through modulation of peroxisome proliferator-activated receptor-gamma (PPAR-γ) activity in vivo and in vitro. RAW264.7 macrophages were treated with different fatty acids, and cell culture supernatants were collected to prepare conditioned media (CM). Different co-culture systems between primary hepatocytes and CM from macrophages were established. A PPAR-γ agonist or antagonist was administered to regulate PPAR-γ activity and macrophage polarization. M1/M2 phenotype markers, inflammatory signaling pathway, and lipid-related genes expression were determined. Wild-type C57BL/6 mice were fed a high-fat diet to induce NAFLD and given rosiglitazone to regulate PPAR-γ activity in vivo. Saturated fatty acids induced M1-polarized macrophages while polyunsaturated fatty acids induced M2-polarized macrophages. M1-polarized macrophages significantly promoted lipid syn...Continue Reading

References

Oct 21, 2006·The Journal of Clinical Investigation·Hang ShiJeffrey S Flier
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Fernando Oneissi MartinezMassimo Locati
Oct 22, 2008·Trends in Endocrinology and Metabolism : TEM·Herbert Tilg, Alexander R Moschen
May 19, 2009·Journal of Hepatology·György Baffy
Oct 31, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Nicolas LanthierIsabelle A Leclercq
May 29, 2010·Circulation Research·Ajay Chawla
Jun 1, 2010·Immunity·Siamon Gordon, Fernando O Martinez
Sep 22, 2010·Nature Immunology·Subhra K Biswas, Alberto Mantovani
Nov 26, 2010·Immunity·Claudio J Villanueva, Peter Tontonoz
Jan 5, 2011·Cell Metabolism·Rebecca G BakerSankar Ghosh
May 9, 2012·Cell Metabolism·Christopher K Glass, Jerrold M Olefsky
May 18, 2013·Nature Reviews. Immunology·Luke A J O'NeillAndrew G Bowie
Jul 9, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jinghong WanCatherine Pavoine
Jan 15, 2014·Journal of Hepatology·Frank Tacke, Henning W Zimmermann
Aug 12, 2014·Gastroenterology·Matthew M Yeh, Elizabeth M Brunt
Apr 29, 2015·Journal of Hepatology·Christopher D Byrne, Giovanni Targher
Dec 29, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Zobair M YounossiMark Wymer
Jan 31, 2016·Gastroenterology·Bin Gao, Hidekazu Tsukamoto
May 31, 2016·Metabolism: Clinical and Experimental·Niki KatsikiChristos S Mantzoros
Mar 18, 2017·Molecular Immunology·Peizhi LiJianping Gong
Aug 29, 2017·Seminars in Liver Disease·Oliver Krenkel, Frank Tacke
May 10, 2018·Nature Reviews. Gastroenterology & Hepatology·Susanne SchusterAriel E Feldstein
Jul 4, 2018·Nature Medicine·Scott L FriedmanArun J Sanyal
Nov 9, 2018·Frontiers in Immunology·Jana HundertmarkFrank Tacke
Nov 30, 2018·Nature Reviews. Gastroenterology & Hepatology·Konstantin KazankovHenning Grønbæk
Dec 18, 2018·Frontiers in Immunology·Elizabeth C StahlBryan N Brown
Mar 6, 2019·Journal of Leukocyte Biology·Priya HandaKris V Kowdley

❮ Previous
Next ❯

Citations

Jul 22, 2021·Journal of Cellular and Molecular Medicine·Aureli LuqueroMaria Borrell-Pages

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.