Regulation of long-chain N-acyl-homoserine lactones in Agrobacterium vitis

Journal of Bacteriology
Guixia Hao, Thomas J Burr

Abstract

Homologs of quorum-sensing luxR and luxI regulatory genes, avsR and avsI, were identified in Agrobacterium vitis strain F2/5. Compared to other LuxI proteins from related species, the deduced AvsI shows the greatest identity to SinI (71%) from Sinorhizobium meliloti Rm1021. AvsR possesses characteristic autoinducer binding and helix-turn-helix DNA binding domains and shares a high level of identity with SinR (38%) from Rm1021. Site-directed mutagenesis of avsR and avsI was performed, and both genes are essential for hypersensitive-like response (HR) and necrosis. Two hypothetical proteins (ORF1 and ORF2) that are positioned downstream of avsR-avsI are also essential for the phenotypes. Profiles of N-acyl-homoserine lactones (AHLs) isolated from the wild type and mutants revealed that disruption of avsI, ORF1, or ORF2 abolished the production of long-chain AHLs. Disruption of avsR reduces long-chain AHLs. Expression of a cloned avsI gene in A. tumefaciens strain NT1 resulted in synthesis of long-chain AHLs. The necrosis and HR phenotypes of the avsI and avsR mutants were fully complemented with cloned avsI. The addition of synthetic AHLs (C(16:1) and 3-O-C(16:1)) complemented grape necrosis in the avsR, avsI, ORF1, and ORF2 muta...Continue Reading

References

Jul 1, 1975·Journal of Bacteriology·B WatsonE W Nester
Nov 1, 1992·Molecular & General Genetics : MGG·J CanadayL Otten
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M D ChiltonE W Nester
Sep 1, 1994·Plant Molecular Biology·P HajdukiewiczP Maliga
Nov 7, 1998·Molecular Plant-microbe Interactions : MPMI·C ChaS K Farrand
Mar 30, 1999·Molecular Microbiology·K A Egland, E P Greenberg
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·Z Q Luo, S K Farrand
Jun 1, 2000·Molecular Plant-microbe Interactions : MPMI·G Hansen
Jun 27, 2000·Journal of Bacteriology·J ZhuS C Winans
Nov 4, 2000·Molecular Plant-microbe Interactions : MPMI·W G MillerS E Lindow
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·J Zhu, S C Winans
Feb 24, 2001·Molecular Plant-microbe Interactions : MPMI·R SantosA Puppo
Aug 29, 2001·FEMS Microbiology Reviews·N A WhiteheadG P Salmond
Sep 7, 2001·Journal of Bacteriology·M Whiteley, E P Greenberg
Nov 10, 2001·Annual Review of Phytopathology·Thomas J. Burr, Leon Otten
Aug 10, 2002·Journal of Bacteriology·Pattarachai KiratisinLuciano Passador
Aug 29, 2002·The EMBO Journal·Alessandro VanniniStefania Di Marco
Oct 11, 2002·The EMBO Journal·Daniela Büttner, Ulla Bonas
Dec 18, 2002·Journal of Bacteriology·Melanie M MarketonJuan E González
Mar 12, 2004·Molecular Biology and Evolution·Emmanuelle Lerat, Nancy A Moran
Jul 31, 2004·Annual Review of Phytopathology·James R Alfano, Alan Collmer
Aug 13, 2004·Molecular Microbiology·Ty A GouldMair E A Churchill

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Citations

Sep 6, 2006·Analytical and Bioanalytical Chemistry·Anne K Dunn, Eric V Stabb
Sep 19, 2012·Analytical and Bioanalytical Chemistry·Tommaso R I CataldiPhilippe Schmitt-Kopplin
Jul 12, 2008·Molecular Plant-microbe Interactions : MPMI·Laura ChalupowiczIsaac Barash
Oct 2, 2009·HFSP Journal·Vittorio Venturi, Sujatha Subramoni
Nov 18, 2015·Molecular Plant-microbe Interactions : MPMI·Desen Zheng, Thomas James Burr
Oct 15, 2009·Phytotherapy Research : PTR·Mira Agnes SzabóJózsef Molnár
Sep 29, 2009·Letters in Applied Microbiology·S SüleT J Burr
Jun 26, 2007·Molecular Microbiology·Jeffrey L BoseEric V Stabb
Apr 23, 2009·Microbiology·Sujatha Subramoni, Vittorio Venturi
Feb 19, 2009·FEMS Microbiology Reviews·Arati V Patankar, Juan E González

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